摘要
雄性不育是作物杂种优势利用的基础。本研究在籼稻93-1160Co-γ辐射诱变突变体库中鉴定了一个普通核雄性不育突变体osgms2。突变体雄性彻底败育,雌性育性正常,其他农艺性状与野生型一致。遗传分析表明雄性不育稳定遗传且为单基因隐性突变,通过与明恢63构建遗传群体将突变基因精细定位于水稻4号染色体长臂的分子标记S3b和4826之间86kb物理区间内。通过测序分析发现定位区间内一编码类成束阿拉伯半乳聚糖蛋白的基因LOC_Os04g48490编码区第118位至126位缺失连续9个碱基,导致氨基酸整码突变。OsGMS2与已报道的OsFLA1为同一基因,但是突变位点不同的新等位突变。粳稻中花11背景下的基因敲除和遗传互补实验证实了该基因的功能。实时荧光定量PCR(qPCR)表达分析模式显示,该基因在水稻各组织均有表达,但在花期和乳熟期种子表达量较高。蛋白同源比对分析发现,该蛋白序列保守,可能在不同物种中发挥重要功能。通过创建OsGMS2-T保持系成功实现了普通核不育系的繁殖。osgms2突变体的发现和基因鉴定为水稻普通核雄性不育研究及杂种优势利用提供了新的材料。
Male sterility is the basis for application of crop heterosis.The common nuclear male sterile mutant osgms2 was isolated from a mutant library created by 60Co-γ-treated indica cultivar 93-11.The male of the mutant was completely aborted,the female was nomal,and other agronomic characters were consistent with those of the wild type.Genetic analyses indicated that the male sterility phenotype was stably controlled by a single recessive gene.The OsGMS2 gene was fine-mapped within the 86 kb physical interval between two molecular markers S3b and 4826 on the long arm of chromosome 4 with a mapping population of osgms2 and Minghui 63.Further sequencing found the gene LOC_Os04g48490,encoding a fasciclin-like arabinogalactan protein,had a 9-bp deletion at position 118 to 126 from start codon of translation,resulting in three codons mutation.The osgms2 gene was a novel allele of OsFLA1.Subsequent gene knockout and genetic complementation experiments in Zhonghua 11 background confirmed the function of the gene.Real-time quantitative PCR(qPCR)analyses showed that the gene was expressed in all tissues,with the highest level at the flowering and immature seed stages.Alignment analyses revealed that the protein sequence was conserved in different species.By creating the OsGMS2-T maintainer,seed production of the common sterile line was achieved.The identification of the allelic mutant osgms2 provides the new materials for the study on rice nuclear male sterility and application of heterosis.
作者
唐杰
龙湍
吴春瑜
李新鹏
曾翔
吴永忠
黄培劲
TANG Jie;LONG Tuan;WU Chun-Yu;LI Xin-Peng;ZENG Xiang;WU Yong-Zhong;HUANG Pei-Jin(Hainan Bolian Technology Co.,Ltd.,Haikou 570125,Hainan,China;College of Tropical Crops,Hainan University,Haikou 570208,Hainan,China)
出处
《作物学报》
CAS
CSCD
北大核心
2023年第8期2025-2038,共14页
Acta Agronomica Sinica
基金
中国水稻研究所水稻生物学国家重点实验室开放课题项目(20190203)资助。
