摘要
【目的】研究眼斑芫菁幼虫肠道共生菌的多样性,并鉴定其相关功能,以探寻眼斑芫菁肠道共生菌在其生长发育中的作用。【方法】分别以人工饲料、蝗卵为食物饲喂眼斑芫菁幼虫,饲养至不同龄期的眼斑芫菁为材料,按相应龄期收集试验所需幼虫,提取眼斑芫菁幼虫肠道细菌基因组DNA,通过16S rDNA全长扩增子测序,分析其肠道细菌的多样性和相对丰度,并探究其肠道共生菌发挥的潜在功能。【结果】获得最终有效序列81 759条,聚类共获得230个OTUs,注释到17个门,27个纲,41个目,71个科,109个属。变形菌门(Proteobacteria)为3个组中的最优势门,各分组样品的优势菌属不相同,劳尔氏菌属为3个组的共同优势菌属。α多样性分析发现,蝗卵饲养的眼斑芫菁二龄幼虫(L.H2)肠道菌群多样性和丰度比四龄幼虫(L.H4)高,且均高于转接入人工饲料饲养的四龄幼虫(L.S4);α与β多样性分析结果均显示眼斑芫菁肠道内共生菌多样性各组内差异大,组间有一定差异,但不同组之间差异不显著(P>0.05)。功能分析结果发现,L.S4在氨基酸代谢、核苷酸代谢、环境适应、免疫系统等方面明显高于L.H4和L.H2,且L.S4的氨基酸代谢比L.H4显著增强(P=0.035)。【结论】明确了眼斑芫菁幼虫肠道菌多样性和群落结构受食物影响,其肠道细菌在代谢功能上能协助宿主适应相应的食物。研究眼斑芫菁幼虫肠道细菌多样性与食物之间的关系,为研究眼斑芫菁肠道微生物变化奠定了基础。
[Objective]The purpose of the study was to explore the diversity of intestinal symbiotic bacteria and identify their related functions in order to explore the role of intestinal symbiotic bacteria in the growth and development of Mylabris cichorii Linnaeus larvae.[Method]The bacterial genome DNA of the larvae was extracted from the artificial diet and locust eggs.The bacterial diversity and relative abundance of the larvae were analyzed by 16S rDNA amplification sequencing,and the bacterial function was analyzed.[Resul]81759 final valid sequences were obtained,and 230 OTUs were obtained by clustering.A total of 17 phyla,27 classes,41 orders,71 families,and 109 genera were annotated.Proteobacteria was the most dominant phylum among the three groups.The dominant bacteria genera of each sample in each group were very difierent,and Lauerella was the common dominant bacteria genus in the three groups.The a diversity analysis showed that the intestinal flora diversity and richness of the second instar larvae(L.H2)were higher than those of the fourth instar larvae(L.H4),and were higher than those of the fourth instar larvae(L.S4)fed with locust eggs.The results of a and B diversity analysis showed that as for the di-veraity of intestinal flora of M.cichorii larvae,there were significant differences among all groups and some differences among groups,but no significant differences among different groups(P>0.05).The results of functional analysis showed that L.S4 were significantly higher than L.H4 and L.H2 in amino acid metabolism,nucleotide metabolism,environmental adaptation and immune system,and the amino acid metabolism of L.S4 were significantly enhanced than that of L.H4(P=0.035).[Conclusion]The diversity and abundance of intestinal flora of M.cichorii larvae are affected by diet,and the intestinal bacteria can assist the host to adapt to the corresponding food in metabolic function.The study of the relationship between the diversity of intestinal bacteria and food of M.cichorii larvae lays the foundation for the study of intestinal microbe of M.cichorii.
作者
易春燕
杜超
杨明
刘昌华
郝员敏
雷应华
刘旭
YI Chun-yan;DU Chao;YANG Ming;LIU Chang-hua;HAO Yuan-min;LEI Ying-hua;LIU Xu(Key Laboratory of Integrated Pest Management of Southwest Crops,Institute of Plant Protection,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China;Baotou Teachers College,Baotou,Inner Mongolia 014000,China)
出处
《西南农业学报》
CSCD
北大核心
2023年第4期868-875,共8页
Southwest China Journal of Agricultural Sciences
基金
四川省科技创新创业苗子工程项目(2020JDRC0128)。
关键词
眼斑芫菁
肠道共生菌
16S
rDNA
多样性
功能预测
tMylabris cichorii Linnaeus
Gut symbiontic microbes
16S rDNA
Diversity
Function prediction
