摘要
目的探讨MD-2基因多态性与早产儿坏死性小肠炎(NEC)的相关性。方法选择2020年1月至2022年12月新生儿重症监护病房收治的确诊早产儿NEC120例(NEC组),采用基因测序方法对坏死性小肠炎患儿进行MD-2基因外显子和启动子功能性多态性区域重测序,将功能性多态点与同期非坏死性小肠炎的患儿120例(非NEC组)进行对照分析。结果NEC组中未检测出功能性多态性的位点,但两组均检测到C-1625G多态位点,且存在C/C和C/G基因型,NEC组C/C基因型频率为54.2%,C/G基因型的频率为45.8%,非NEC组C/C基因型频率为65.0%,C/G基因型频率为35.0%,两组比较差异无统计学意义(P>0.05)。手术组C/C基因型频率为50.0%,C/G基因型频率为50.0%,与非NEC组比较,差异有统计学意义(P<0.05)。NEC组G等位基因型频率为22.9%,非NEC组G等位基因型频率为11.7%,两组比较差异无统计学意义(P>0.05);手术组G等位基因型频率为10.4%,与非NEC组比较,差异有统计学意义(P<0.05)。手术组G等位基因频率与非手术组比较,差异无统计学意义(P>0.05)。结论新生儿坏死性小肠炎发病与MD-2基因的外显因子的基因多态性无关,但疾病的严重程度可能与G等位基因有关。
Objective To investigate the relationship between MD-2 gene polymorphism and neonatal necrotizing enteritis(NEC)in premature infants.Methods 120 premature infants with neonatal necrotizing enterocolitis(the NEC group)admitted in the neonatal intensive care unit of our hospital from 2020 to 2022 were selected to participate in this study.The functional polymorphism regions of MD-2 gene exons and promoters were re-sequenced by gene sequencing method in children with necrotizing enterocolitis,and the functional polymorphisms were compared with 120 children with nonnecrotizing enterocolitis in the same period(the non-NEC group).Results No functional polymorphic site was detected in the NEC group,but c-1625G polymorphic site was detected in the necrotizing colitis group and the non-necrotizing colitis group,and there were two genotypes of C/C and C/g.The frequency of C/C genotype in NEC group was 54.2%,the frequency of C/g genotype was 45.8%,the frequency of C/C genotype in the non-NEC group was 65.0%,and the frequency of C/g genotype was 35.0%.The difference between the two groups was not statistically significant(P>0.05).The frequency of C/C genotype and C/g genotype in the operation group were 50.0%and 50.0%,respectively.Compared with the non-NEC group,the difference in results was statistically significant(P<0.05).The frequency of G allele in NEC group was 22.9%,and the frequency of G allele in the non-NEC group was 11.7%.The results of the two groups were different but did not meet the statistical criteria(P>0.05).The frequency of G allele in the operation group was 10.4%,which was statistically significant compared with that in the non-NEC group(P<0.05).The G allele frequency of the surgical group was higher than that of the non-surgical group,but the results were different but did not meet the statistical criteria(P>0.05).Conclusion The incidence of neonatal necrotizing colitis is not related to the gene polymorphism of the exon of MD-2 gene,but the severity of the disease may be related to the G allele.
出处
《浙江临床医学》
2023年第5期667-669,共3页
Zhejiang Clinical Medical Journal
基金
浙江省医药卫生科技计划项目(2020KY879)
浙江省省市共建医学扶植重点学科(2010-S04)。
