摘要
目的建立RP-HPLC法测定眩晕宁颗粒中3,5-O-二咖啡酰基奎宁酸、23-乙酰泽泻醇B和β-蜕皮甾酮的测定方法。方法采用Capcell Pak UG C18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈–0.5%磷酸溶液,梯度洗脱;检测波长:348 nm(3,5-O-二咖啡酰基奎宁酸)、250 nm(23-乙酰泽泻醇B)、208 nm(β-蜕皮甾酮);体积流量:1.0 mL/min;柱温:28℃;进样量:10μL。结果3,5-O-二咖啡酰基奎宁酸、β-蜕皮甾酮和23-乙酰泽泻醇B分别在0.0767~7.6700、0.0983~9.8300、0.0197~1.9700μg/mL线性良好,平均回收率分别为100.05%、98.33%、99.42%,RSD值分别为0.7%、1.2%、1.3%。结论方法具有前处理简单、分析时间短、检测结果准确等优点,适用于眩晕宁颗粒的质量控制。
Objective To establish an RP-HPLC method for the determination of 3,5-O-dicaffeyl quinic acid,23-acetylalisol B,andβ-ecdysteroid in Xuanyunning Granules.Methods The separation was carried out on Capcell Pak UG C18 column(250 mm×4.6 mm,5μm).The mobile phase was acetonitrile-0.5%phosphoric acid solution with gradient elution.The detection wavelength was set at 348 nm(3,5-O-dicaffeyl quinic acid),250 nm(23-acetylalisol B),and 208 nm(β-ecdysterone).The flow rate was 1.0 mL/min,temperature of column was set at 28℃,and the volume of injection was 10μL.Results 3,5-O-dicaffeyl quinic acid,23-acetylalisol B,andβ-ecdysteroid showed good linear relationships in concentration ranges of 0.0767—7.6700,0.0983—9.8300,and 0.0197—1.9700μg/mL,and their average recoveries were 100.05%,98.33%,and 99.42%with RSD values of 0.7%,1.2%,and 1.3%,respectively.Conclusion The method has the advantages of simple pretreatment,short analysis time,and accurate detection results,and is suitable for the quality control of Xuanyunning Granules.
作者
王泳
汪燕
黄灿
陶贤琦
邢薇薇
齐腊梅
WANG Yong;WANG Yan;HUANG Can;TAO Xian-qi;XING Wei-wei;QI La-mei(Department of Pharmacy Management,Anqing Municipal Hospital,Anqing 246003,China;Anhui Institute for Food and Drug Inspection,Hefei 230051,China)
出处
《现代药物与临床》
CAS
2023年第5期1109-1112,共4页
Drugs & Clinic
基金
安徽医科大学校科研基金项目(2020xkj241)。
