摘要
目的探讨吡非尼酮(PFD)对急性胰腺炎(AP)大鼠胰腺损伤的影响及其作用机制。方法将32只SPF级SD雄性大鼠随机分为对照组、AP组及PFD低、高剂量组,每组8只。AP组及PFD低、高剂量组均成功复制AP模型,PFD低、高剂量组分别给予PFD 50和150 mg/(kg·d)灌胃治疗24 h,AP组、对照组大鼠则灌胃等量0.5%羧甲基纤维素钠。干预完成后,测定各组大鼠腹水量、血清淀粉酶(AMY)、血清炎症因子水平,观察各组大鼠胰腺组织病理变化并进行病理评分,比较各组大鼠胰腺组织Toll样受体4/髓样细胞分化蛋白88(TLR4/MYD88)通路蛋白的表达。结果与对照组比较,AP组大鼠腹水量增加(P<0.05),血清AMY、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平、病理评分、Toll样受体4(TLR4)和MYD88蛋白相对表达量表达升高(P<0.05);与AP组比较,PFD低、高剂量组腹水量减少(P<0.05),血清AMY、IL-6、TNF-α水平、病理评分、TLR4和MYD88蛋白相对表达量降低(P<0.05);与PFD低剂量组比较,高剂量组腹水量减少(P<0.05),血清AMY、IL-6、TNF-α水平、病理评分、TLR4和MYD88蛋白相对表达量降低(P<0.05)。结论PFD对AP大鼠胰腺损伤具有保护作用,其作用机制可能与抑制TLR4/MYD88信号通路活化有关。
Objective To investigate the effect of pirfenidone(PFD)on pancreatic injury in rats with acute pancreatitis(AP)and its mechanism of action.Methods Thirty-two SPF male SD rats were randomly divided into control group,AP group and low-and high-dose PFD groups,with 8 rats in each group.AP models were successfully established in the AP group,and low-and high-dose PFD groups.Rats in the low-and high-dose groups were intragastrically administrated with PFD at doses of 50 mg/(kg·d)and 150 mg/(kg·d)for 24 hours,respectively,while those in the AP group and the control group were given the same amount of 0.5%sodium carboxymethyl cellulose.After the intervention,the volume of ascites and serum levels of amylase(AMY)and inflammatory factors were measured,and the histopathological changes of the pancreatic tissues were observed and scored in each group.Besides,the expressions of proteins associated with the Toll-like receptor 4/myeloid differentiation 88(TLR4/MYD88)pathway in the pancreatic tissues of rats in each group were also compared.Results The volume of ascites,serum levels of AMY,IL-6 and TNF-α,the histopathological scores of the pancreatic tissues,and the protein expressions of TLR4 and MYD88 in the AP group were higher than those in the control group(P<0.05),while they were lower in the low-and high-dose PFD groups than those in the AP group(P<0.05)and were even lower in the high-dose PFD group than those in the low-dose PFD group(P<0.05).Conclusions PFD has a protective effect on pancreatic injury in rats with AP,and its mechanism may be related to the inhibition of TLR4/MYD88 signaling pathway activation.
作者
黄丽
刘静
Huang Li;Liu Jing(Department of Gastroenterology,Affiliated Hospital of Chengdu University,Chengdu,Sichuan 610081,China;Department of Gastrointestinal Surgery,Suining Central Hospital,Suining,Sichuan 629099,China)
出处
《中国现代医学杂志》
CAS
北大核心
2023年第12期1-5,共5页
China Journal of Modern Medicine
基金
四川省科技计划项目(No:2020YFS0490)。
