摘要
目的探究沉默信息调节因子1(silent information regulator 1,SIRT1)、成纤维细胞活化蛋白-α(fibroblast activation protein-α,FAP-α)、半胱氨酸天冬氨酸蛋白酶3(cysteine aspartase-3,caspase-3)在结直肠癌中的表达及相关性。方法收集2021年4月至2022年4月整理归档较为完整的45例为结直肠癌组织石蜡标本,另外选取45例为距离癌组织边缘5 cm的癌旁组织石蜡标本。采取免疫组化法检测SIRT1、FAP-α、caspase-3水平表达,分析SIRT1、FAP-α、caspase-3在结直肠癌中的表达变化及与预后的相关性。结果与癌旁组织相比,结直肠癌组织中SIRT1、FAP-α、caspase-3表达量均有所升高,差异有统计学意义(P<0.05)。结直肠癌组织中SIRT1、FAP-α、caspase-3表达与结直肠癌患者的年龄、性别、肿瘤位置、远处转移无关,与淋巴结转移、肿瘤的直径、组织学类别、浸润程度、TNM分期、脉管瘤栓、分化程度有关相关,差异有统计学意义(P<0.05)。SIRT1、FAP-α、caspase-3之间Pearson相关性分析显示,SIRT1、FAP-α之间呈正相关(r=0.534,P=0.001);FAP-α、caspase-3之间呈正相关(r=0.556,P=0.001);SIRT1、caspase-3之间呈正相关(r=0.585,P=0.001)。ROC曲线显示,三项联合对结直肠癌的诊断价值高于SIRT1、FAP-α、caspase-3单项诊断(P=0.001)。结论结直肠癌患者机体内SIRT1、FAP-α、caspase-3水平表达上升,可看出其水平变化与结直肠癌患者产生病症有密切的关联,因此三者可作为预测结直肠癌患者治疗的重要依据。
Objective To explore the expression levels of silent information regulator 1(SIRT1),fibroblast activation protein-α(FAP-α),and cysteine aspartase-3(caspase-3)in colorectal cancer(CRC)and their correlation.Methods A total of 45 paraffin-embedded CRC tissues were collected from April 2021 to April 2022,and another 45 paraffin-embedded specimens surgically collected from 5cm away from the edge of cancer tissues were selected as controls.The expression levels of SIRT1,FAP-αand caspase-3 were determined by immunohistochemistry,and their correlation with the prognosis of CRC was analyzed.Results Compared with those of controls,SIRT1,FAP-α,and caspase-3 were significantly upregulated in CRC tissues(P<0.05).Their expressions in CRC tissues were not correlated with the age,sex,tumor location,and distant metastasis,but significantly correlated with lymph node metastasis,tumor diameter,histological category,infiltration degree,TNM staging,vascular tumor embolus,and differentiation degree(P<0.05).The Pearson correlation analysis showed a positive correlation between the expression levels of SIRT1 and FAP-α(r=0.534,P=0.001).A positive correlation between the expression levels of FAP-αand caspase-3 was identified(r=0.556,P=0.001),and meanwhile,a positive correlation was identified between the expression levels of SIRT1 and caspase-3(r=0.585,P=0.001).The ROC curve showed that the value of the combination detections of SIRT1,FAP-αand caspase-3 had a significantly higher diagnostic value in CRC than that of a single detection of either of them(P=0.001).Conclusion SIRT1,FAP-αand caspase-3 are upregulated in CRC patients,which are significantly correlated with the disease condition.The combination detection of them provides important references for predicting the prognosis of CRC.
作者
王春华
杜刚
杜永刚
邓亨怡
WANG Chunhua;DU Gang;DU Yonggang(Department of Gastrointestinal Surgery,the People’s Hospital of Pengzhou,Sichuan,Pengzhou 610097,China;不详)
出处
《河北医药》
CAS
2023年第12期1770-1774,共5页
Hebei Medical Journal
基金
浙江省医药卫生科技计划项目(编号:2019RC283)。
