摘要
为研究富硒乳酸菌(SL)对犬流感病毒(CIV)感染诱导肺损伤的保护作用,选取90只6~7周龄的C57BL/6小鼠,随机分为对照组,CIV感染组(CIV组),CIV+乳酸菌组(CIV+L组),CIV+亚硒酸钠组(CIV+SS组)和CIV+富硒乳酸菌组(CIV+SL组),每组3个重复,每个重复6只小鼠。各组分别饲喂上述不同日粮,1周后除对照组外,其他各组均用H3N2亚型CIV毒株(JS/10)滴鼻,攻毒后1周解剖各组小鼠,取肺组织,计算肺指数,观察肺组织形态结构,HE染色检测肺组织病理变化,检测肺组织中病毒含量,检测抗氧化指标。结果显示:CIV感染后肺指数显著升高(P<0.05),肺组织水肿和出血,炎性细胞浸润,肺泡间隔增厚;肺组织检测到病毒M mRNA及NP蛋白;肺脏中超氧化物歧化酶(SOD)活性和总抗氧化能力(T-AOC)水平降低(P<0.05),丙二醛(MDA)含量升高(P<0.05)。与CIV组相比,CIV+L组、CIV+SS组和CIV+SL组肺指数显著降低(P<0.05),肺组织病变程度减轻,炎性细胞浸润明显减轻,肺泡间隔增厚减少;肺组织病毒M mRNA及NP蛋白水平显著降低(P<0.05);肺脏中SOD活性和T-AOC水平显著升高(P<0.05),MDA含量降低(P<0.05)。CIV+SL组与CIV+L和CIV+SS组相比,CIV+SL组肺指数显著降低(P<0.05),CIV+L和CIV+SS组表现为中等程度的的水肿和出血、炎性细胞浸润和肺泡间隔增厚;肺组织病毒M mRNA及NP蛋白水平显著降低(P<0.05);肺脏中SOD活性和T-AOC水平显著升高(P<0.05),MDA含量降低(P<0.05)。综上,富硒乳酸菌对H3N2亚型CIV感染引起的肺损伤具有较好的保护作用,其作用可能与抑制病毒感染与提高抗氧化能力有关。
In order to determine the protective effects of selenium-enriched Lactobacillus on lung injury induced by canine influenza virus infection,a total of ninety 6-7-week-old C57BL/6 mice were selected and randomly divided into the control group,the canine influenza virus-infection group(CIV group),the CIV+Lactobacillus group(CIV+L group),the CIV+sodium selenite group(CIV+SS group)and the CIV+selenium-enriched lactobacillus group(CIV+SL group),with 3 replicates in each group and 6 mice in each replicate.The mice in different groups were fed with the above mentioned different diets,respectively.Except the control group,animals in all the other groups were infected by intranasal inoculation with the H3N2 subtype CIV strain(JS/10)after a week of trial feeding.These rodents were slaughtered and lung tissues were collected from them after one week of trial feeding.The lung index was calculated,the morphological structures of the lung tissues were observed,the pathological changes in the lung tissue were detected by HE staining,and the virus content and the changes in the antioxidant capacity index were detected in the lung tissues.After CIV-infection,the lung index was significantly increased(P<0.05),the lung tissues exhibited edema and hemorrhage,inflammatory cell infiltration in the tissues was obvious,and alveolar septum thickening in them was observed.The activity of SOD(P<0.05)and the level of T-AOC(P<0.05)in the lung tissues were decreased,and the content of MDA in them was increased(P<0.05).Viral M mRNA and NP protein were detected in the lung tissues.After supplementing lactic acid bacteria,sodium selenite,and selenium-enriched Lactobacillus,the lung index was significantly decreased(P<0.05),the degrees of lung tissue lesions,inflammatory cell infiltration,and alveolar septum thickening were significantly reduced,the levels of viral M mRNA and NP protein in the lung tissues were significantly decreased(P<0.05)and the SOD activity(P<0.05)and the T-AOC level(P<0.05)therein were increased,but the MDA content in there was decreased(P<0.05),as compared with the CIV group.Compared with the CIV+L and CIV+SS groups,the lung index of the CIV+SL group was significantly decreased(P<0.05),and the CIV+L and CIV+SS groups showed a moderate degree of hemorrhage and edema,inflammatory cell infiltration,and alveolar septum thickening.The levels of viral M mRNA and NP protein in the lung tissues were significantly decreased(P<0.05),the SOD activity and the T-AOC level in the lung were significantly increased(P<0.05),and the MDA content was decreased(P<0.05).To sum up,selenium-enriched Lactobacillus had a good protective effect on lung injury caused by H3N2 subtype CIV infection,and its effect might be related to the reduction in virus content and improvement of antioxidant capacity in the lung.
作者
王孟孟
刘水平
唐江渝
李金燕
石蕊
吴小马
刘丹丹
甘芳
刘云欢
陈兴祥
黄克和
WANG Mengmeng;LIU Shuiping;TANG Jiangyu;LI Jinyan;SHI Rui;WU Xiaoma;LIU Dandan;GAN Fang;LIU Yunhuan;CHEN Xingxiang;HUANG Kehe(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;Jiangsu Xietong Pharmaceutical Bioengineering Co.,Ltd.,Nanjing 211164,China)
出处
《畜牧与兽医》
CAS
北大核心
2023年第6期118-123,共6页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金联合基金重点项目(U22A20524)。
关键词
富硒乳酸菌
犬流感病毒
感染
肺损伤
抗氧化能力
selenium-enriched Lactobacillus
canine influenza virus
infection
lung injury
antioxidant ability
