摘要
目的 探讨三七总皂苷、莪术醇联用对抗结肠癌细胞株HCT116和HT29的效果及其可能的机制。方法 采用体外培养HCT116及HT29结肠癌细胞系模型,分别给予三七总皂苷(50μmol/L)(三七总皂苷组)、莪术醇(50μmol/L)(莪术醇组)及联合用药(50μmol/L三七总皂苷+50μmol/L莪术醇)(联合组)进行处理,并将细胞培养24 h。以5-FU为阳性对照设置阳性对照组,同时设置空白对照组(只加细胞和培养液)。体外培养24、48 h后,统计细胞抑制率、细胞凋亡及细胞周期情况,采用免疫细胞化学法检测Ang-2和caspase-3蛋白的表达。结果 人结肠癌细胞经三七总皂苷、莪术醇以及联合处理后,细胞生长受到不同程度的抑制,联合组对HCT116和HT29的抑制率明显高于单药的应用,差异有统计学意义(P<0.05),且抑制程度随着时间的增加而增强;联合组干预48 h后人结肠癌HCT116和HT29细胞凋亡率明显高于各单用组,差异有统计学意义(P<0.05);联合组干预24 h后,HCT116、HT-29细胞发生变化,G1期细胞数增加,S期细胞数明显减少,差异有统计学意义(P<0.05);联合组干预24 h后,HCT116和HT29细胞中Ang-2的表达水平明显降低,caspase-3的表达水平则明显升高,差异有统计学意义(P<0.05)。结论 三七总皂苷、莪术醇联用能显著增强抗结肠癌作用,其机制可能是通过抑制细胞增殖,并促进细胞凋亡的双重作用。
Objective To investigate the effects and mechanism of Panax notoginseng saponins(PNS)and curcumenol against the colon cancer cell lines HCT116 and HT29.Methods HCT116 and HT29 colon cancer cell line models were cultured and treated with 50μmol/L PNS,50μmol/L curcumenol,and 50μmol/L PNS+50μmol/L curcumenol,respectively,for 24 h.A positive control(5-fluorouracil,5-FU)group and a blank control(only cells and medium)group were designed.After in vitro culture for 24 h and 48 h,the inhibition rate,apoptosis,and cell cycle were recorded,and immunocytochemistry was employed to measure the expression of angiotensin-2(Ang-2)and caspase-3.Results The treatment with PNS and/or curcumenol inhibited the growth of HCT116 and HT29 cells to different degrees,and the PNS+curcumenol group had higher inhibition rate than the PNS or curcumenol group(P<0.05).Furthermore,the inhibitory effect enhanced over time.The apoptosis rate of HCT116 and HT29 cells treated with PNS+curcumenol for 48 h was higher than that of the cells treated with PNS or curcumenol(P<0.05).The treatment with PNS+curcumenol for 24 h increased the cells in G1 phase and reduced the cells in S phase(P<0.05).Furthermore,the treatment with PNS+curcumenol for 24 h down-regulated the expression level of Ang-2 and up-regulated the expression level of caspase-3 in HCT116 and HT29 cells(P<0.05).Conclusion The combination of PNS and curcumenol can exert the anti-colon cancer effect by inhibiting cell proliferation and promoting apoptosis.
作者
孙成栋
高淑芳
刘佳
张坚
张雪梅
SUN Cheng-dong;GAO Shu-fang;LIU Jia;ZHANG Jian;ZHANG Xue-mei(Department of Infectious Diseases,Beijing Jishuitan Hosipital,Beijing 100035;Department of Ophthalmology,Weihai Municipal Hospital affiliated to Shandong University,Weihai Shandong 264200)
出处
《世界中西医结合杂志》
2023年第6期1088-1092,1128,共6页
World Journal of Integrated Traditional and Western Medicine
基金
吴阶平医学基金会专项资助基金立项(320.6750.19089-98)。
关键词
三七总皂苷
莪术醇
结肠癌
细胞增殖
细胞凋亡
Panax notoginseng Saponins
Curcumenol
Colon Cancer
Cell Proliferation
Cell Apoptosis
