摘要
目的探讨原发性舍格伦综合征(primary Sjogren’s syndrome,pSS)患者血清中miR-193b表达变化以及临床意义。方法选取2015年9月~2020年9月在黄石市中心医院治疗的98例pSS患者作为病例组,45例健康体检者作为对照组。根据患者病情严重程度分为轻度组(n=31)、中度组(n=43)和重度组(n=24)。收集临床资料,实时荧光定量PCR法检测血清中miR-193b表达量,采用Pearson相关分析分析pSS患者血清中miR-193b表达量和临床指标间相关性,采用受试者工作特征(ROC)曲线分析血清中miR-193b表达量对pSS患者诊断价值。结果pSS患者血清中miR-193b表达量在对照组、pSS轻度组、pSS中度组和pSS重度组依次升高(1.79±0.311,1.91±0.21,2.46±0.25,3.11±0.22),差异有统计学意义(F=165.948,P<0.001),且pSS重度组>pSS中度组>pSS轻度组>对照组(t=10.720,9.924,2.029,均P<0.05);Pearson相关分析结果显示,pSS患者血清中miR-193b表达量与B细胞比例、ESR,IgG,ESSDAI评分和ESSPRI评分呈正相关(r=0.432,0.554,0.448,0.356和0.361,均P<0.05),而与白细胞数、唾液流率和泪流率呈负相关(r=-0.215,-0.323,-0.369,均P<0.05);ROC曲线分析结果显示,血清中miR-193b表达量在预测pSS发生时曲线下面积为0.864(95%CI:0.805~0.923),当血清中miR-193b表达量为2.06时,灵敏度和特异度分别为78.57%,82.22%。结论miR-193b在pSS患者血清中表达量升高,与pSS病情轻重和患者腺体分泌有关,检测血清miR-193b有助于诊断pSS。
Objective To investigate the change and significance of the expression of miR-193b in the serum of patients with primary Sjogren's syndrome(pSS).Methods A total of 98 cases of pSS patients treated in Huangshi Central Hospital were selected from September 2015 to September 2020,and 45 healthy subjects were served as the control group.According to the severity of the patient’s condition,patients were divided into mild group(n=31),moderate group(n=43)and severe group(n=24).The clinical data was collected.The expression of miR-193b in the serum was detected by using real-time fluorescent quantitative PCR.Pearson correlation analysis was used to analyze the correlation between the expression of miR-193b in serum of pSS patients and clinical indicators,and receiver operating characteristic curve(ROC)was used to analyze the diagnostic value of the expression of miR-193b in the serum for pSS patients.Results The relative expression levels of miR-193b in the serum of pSS patients in the control group,mild group,moderate group and severe group were 1.79±0.31,1.91±0.21,2.46±0.25 and 3.11±0.22,repectively,and the difference was statistically significant(F=165.948,P<0.001),and the severe group>moderate group>mild group>control group(t=10.720,9.924,2.029,all P<0.05).Pearson correlation analysis results showed that the expression level of miR-193b in the serum of pSS patients was positively correlated with the proportion of B cells,ESR,IgG,ESSDAI score and ESSPRI score(r=0.432,0.554,0.448,0.356 and 0.361,all P<0.05),while it was negative correlated with the number of white blood cells,saliva flow rateand tear flow rate(r=-0.215,-0.323,-0.369,all P<0.05).ROC curve analysis results showed that the area under the curve for the expression level of miR-193b in the serum when predicting the occurrence of pSS was 0.864(95%CI:0.805~0.923).When the expression level of miR-193b in the serum was 2.06,the sensitivity and the specificity were 78.57%,82.22%,respectively.Conclusion The expression level of miR-193b in the serum of pSS patients was elevated,which was related to disease activity and gland function.It is expected to be a serum marker for early diagnosis of pSS patients.
作者
石娴
傅曼妮
解翠林
SHI Xian;FU Manni;XIE Cuilin(Department of Dermatology,Huangshi Central Hospital of Eastern Hubei Medical Group/Affiliated Hospital of Hubei Institute of Technology,Hubei Huangshi 435000,China)
出处
《现代检验医学杂志》
CAS
2023年第4期126-130,共5页
Journal of Modern Laboratory Medicine
