摘要
目的 检测过敏性鼻炎(AR)患者外周血中细胞分裂周期相关蛋白5(CDCA5)、微小RNA-146a(miR-146a)及鼻腔分泌物中S100钙结合蛋白A7(S100A7)的水平,分析上述3项指标与AR病情严重程度的关系及其对AR的临床诊断价值。方法 回顾性选取2019年7月至2022年3月于延安市人民医院就诊的83例AR患者作为观察组,另选取同期在该院体检且无过敏性疾病和呼吸道相关疾病的健康人50例作为对照组。采用实时荧光定量聚合酶联反应和酶联免疫吸附试验分别检测两组受试者外周血中CDCA5、miR-146a水平及鼻腔分泌物中S100A7水平;采用Spearman相关分析CDCA5、miR-146a、S100A7与AR病情严重程度的相关性,绘制受试者工作特征(ROC)曲线分析CDCA5、miR-146a、S100A7对AR的诊断效能。结果 观察组患者CDCA5水平高于对照组,miR-146a和S100A7水平低于对照组,差异均有统计学意义(P<0.05)。CDCA5水平与AR病情严重程度呈正相关(r=0.637,P<0.05);miR-146a、S100A7水平与AR病情严重程度呈负相关(r=-0.596、-0.605,P<0.05)。CDCA5、miR-146a、S100A7单独诊断AR的曲线下面积(AUC)分别为0.778、0.784、0.806,而3项指标联合诊断AR的AUC为0.897、灵敏度为87.9%、特异度为83.6%。结论 CDCA5、miR-146a、S100A7水平与AR患者病情严重程度关系密切,3项指标联合检测能提高对AR的临床诊断效能,对评估患者病情及指导临床诊治具有重要意义。
Objective To detect the expression levels of cell division cycle associated protein 5(CDCA5)and micro RNA-146a(miR-146a)in peripheral blood and S100 calcium-binding protein A7(S100A7)in nasal secretion of patients with allergic rhinitis(AR),and analyze the relationship between the above three indexes and the severity of AR and their clinical diagnostic values for AR.Methods A total of 83 AR patients treated in Yan′an City People′s Hospital from July 2019 to March 2022 were retrospectively selected as the observation group,while another 50 healthy subjects who underwent physical examination in the hospital during the same period were selected as the control group.The expression levels of of CDCA5 and miR-146a in peripheral blood and S100A7 in nasal secretion in the two groups were detected by real-time quantitative polymerase chain reaction and enzyme linked immunosorbent assay,respectively.The correlations between CDCA5,miR-146a and S100A7 and the severity of AR were analyzed,and the clinical diagnostic values of their expression detection for AR were evaluated.Results The expression level of CDCA5 in the observation group was higher than that in the control group,and the expression levels of miR-146a and S100A7 were lower than those in the control group,with statistically significant difference(P<0.05).The expression level of CDCA5 was positively correlated with the severity of AR(r=0.637,P<0.05),while the expression levels of miR-146a and S100A7 were negatively correlated with the severity of AR(r=-0.596,-0.605,P<0.05).The area under the receiving operating curve(AUC)of CDCA5,miR-146a and S100A7 alone for the diagnosis of AR was 0.778,0.784,and 0.806,respectively,while the AUC of the combined detection of the three indexes were 0.897,with sensitivity of 87.9%and specificity of 83.6%.Conclusion The expression levels of CDCA5,miR-146a and S100A7 are closely related to the severity of AR patients,and the combined detection of the three indexes can improve the clinical diagnostic efficiency of AR,which is of great significance to evaluate the patient′s condition and guide clinical diagnosis and treatment.
作者
石昊
薛媛
安丽
闫娟
拓明祥
李二乐
SHI Hao;XUE Yuan;AN Li;YAN Juan;TUO Mingxiang;LI Erle(Otolaryngology-Head and Neck Surgery,Yan′an City People′s Hospital,Yan′an,Shaanxi 716000,China)
出处
《检验医学与临床》
CAS
2023年第15期2170-2173,2177,共5页
Laboratory Medicine and Clinic
基金
陕西省重点研发计划项目(2021SF-264)。
