摘要
以药食同源中药材人参为原材料,通过超声辅助热水法提取人参多糖(Ginseng polysaccharides,GPS),经酶解法去除淀粉和蛋白质,再经DEAE-52纤维素柱层析分离纯化得到精制人参多糖(GPS-1)。通过高效凝胶渗透色谱、红外光谱和核磁共振波谱测定了GPS-1的相对分子质量、单糖构成和结构特征,另外研究了GPS-1对人皮肤成纤维细胞(Human skin fibroblasts,HFF-1)存活率和HFF-1中活性氧(Reactive oxygen,ROS)、丙二醛(Malondialdehyde,MDA)、谷胱甘肽过氧化物酶(Glutathion peroxidase,GSH-Px)、超氧化物歧化酶(Superoxide dismutase,SOD)、金属蛋白酶1(Metalloproteinase 1,MMP-1)和金属蛋白酶9(Metalloproteinase 9,MMP-9)的影响。结果表明,GPS-1为均一性多糖,多糖纯度为95.13%,重均分子量为2.104 kDa,由α-D-葡萄糖、α-D-半乳糖醛酸、α-D-阿拉伯糖、α-D-岩藻糖、α-D-核糖、β-D-甘露糖和β-D-阿拉伯糖构成。GPS-1可保护UVB照射下的HFF-1细胞活性,质量浓度为200μg/mL的GPS-1溶液即可显著抑制MMP-1和MMP-9的表达(P<0.01),显著提升SOD和GSH-Px的含量(P<0.01),显著降低ROS和MDA的含量(P<0.01),为人参多糖的分离纯化以及在开发抗衰老产品的应用提供了科学依据。
Ginseng polysaccharide(GPS)was obtained from ginseng,a traditional Chinese medicinal material,through an efficient extraction method in this work.Namely,the extracts of ginseng were first collected by ultrasonic assisted extraction of hot water,then purified through enzymolysis to remove the starch and protein,and finally refined by DEAE-52 cellulose column chromatography to get the desired ginseng polysaccharide(GPS-1).The molecular weight,monosaccharide composition and structure of GPS-1 were determined by high performance gel permeation chromatography,infrared spectroscopy and nuclear magnetic resonance spectroscopy.In addition,the role of GPS-1 in human skin fibroblasts(HFF-1)survival rate and the effect on reactive oxygen species(ROS),malondialdehyde(MDA),glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),metalloproteinase 1(MMP-1)and metalloproteinase 9(MMP-9)were studied.The results showed that GPS-1 was a homogeneous polysaccharide with a purity of 95.13%and an average molecular weight of 2.104 kDa,which was composed ofα-D-glucose,α-D-galacturonic acid,α-D-arabinose,α-D-fucose,α-D-ribose,β-D-mannose andβ-D-arabinose.Moreover,the cell experiments confirmed that GPS-1 could protect HFF-1 cells from UVB irradiation,and the expression of MMP-1 and MMP-9 could be significantly inhibited by GPS-1 solution at the concentration of 200μg/mL(P<0.01).Further study revealed that GPS-1 could also significantly increase SOD and GSH-Px content(P<0.01)and decrease the contents of ROS and MDA(P<0.01).These results would provide a scientific basis for the isolation and purification of ginseng polysaccharide and its application in the development of anti-aging products.
作者
聂梅
黎鹏
汤静洁
张平军
黄冬婷
NIE Mei;LI Peng;TANG Jingjie;ZHANG Pingjun;HUANG Dongting(Institute of Biological and Medical Engineering,Guangdong Academy of Sciences,Guangzhou 510316,China;Guangdong Province Engineering Research Center for Green Technology of Sugar Industry,Guangzhou 510316,China)
出处
《食品工业科技》
CAS
北大核心
2023年第16期412-419,共8页
Science and Technology of Food Industry
基金
广东省科学院发展专项资金项目(2020GDASYL-20200103069)
揭阳市科技计划项目(2019089)。
