摘要
本试验旨在研究芪枣提取物对余干乌骨鸡蛋品质、脂质代谢和血清抗氧化指标的影响。试验选用240日龄体况良好的余干乌骨鸡120只,随机分成4个组,每个组6个重复,每个重复5只鸡。对照组饲喂基础日粮,试验组在基础日粮中分别添加100、200和400 mg/kg芪枣提取物,试验期为56 d。结果表明:(1)余干乌骨鸡蛋壳黄度随芪枣提取物水平的增加呈线性增加(P<0.05),哈氏单位、蛋黄指数和蛋壳红度呈二次曲线变化(P<0.05),其他蛋品质指标各组之间无显著差异。(2)饲粮中添加芪枣提取物对余干乌骨鸡血清的甘油三酯、总胆固醇、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇无显著差异。(3)芪枣提取物对余干乌骨鸡蛋黄胆固醇含量无影响。(4)余干乌骨鸡卵巢中VLDLR和肝脏中HMGR基因表达水平随芪枣提取物添加水平的增加呈二次曲线变化(P<0.05)。(5)余干乌骨鸡血清中CAT、T-AOC和MDA含量随芪枣提取物水平的增加呈二次曲线变化(P<0.05)。由此可见,饲粮中添加芪枣提取物能够提高余干乌骨鸡的抗氧化能力,并通过调节血清中脂肪代谢和抑制HMGR、VLDLR基因表达水平来调控蛋黄胆固醇的含量,最佳添加量是200 mg/kg。
The purpose of this experiment was to study the effects of Qizao extract on egg quality,lipid metabolism and ser-um antioxidant indexes of Yugan silky chickens.120240-dayold Yugan silky chickens in good body condition were selected for the experiment and randomly divided into 4 groups with 6 replicates in each group and 5 chickens in each replicate.The con-trol group was fed the basal diet.The experimental group added 100,200 and 400 mg/kg of Qizao extract to the basal diet,re-spectively.The test period was 56 days.The results showed that:(1)The eggshell yellowness of Yugan silky chicken increased linearly with the increase of Qizao extract(P<0.05),and Haugh unit,egg yolk index and eggshell redness changed quadrati-cally(P<0.05),there were no significant differences among the other egg quality indexes among the groups.(2)Adding Qizao extract to diet had no significant difference on serum triglyceride,total cholesterol,high-density lipoprotein cholesterol and low-density lipoprotein cholesterol of Yugan silky chicken.(3)Qizao extract had no effect on the cholesterol content of Yugan black-bone egg yolk.(4)The expression levels of VLDLR in Yugan silky chicken ovary and HMGR in liver showed a quad-ratic curve with the increase of Qizao extract(P<0.05).(5)The content of CAT,T-AOC and MDA in Yugan silky chick-en serum showed a quadratic curve with the increase of Qizao extract(P<0.05).It can be seen that adding Qizao extract to the diet can improve the antioxidant capacity of Yugan silky chickens,and regulate the content of egg yolk cholesterol by regulating the fat metabolism in serum and inhibiting the ex-pression levels of HMGR and VLDLR genes.The optimal addition amount is 200 mg/kg.
作者
吕宏伟
贺亚媚
唐艳强
李思明
范志勇
LV Hongwei;HE Yamei;TANG Yanqiang;LI Siming;FAN Zhiyong(Hanzhong Vocational and Technical College,Hanzhong Shaanzi 723000,China;College of Animal Science and Technology,Hunan Agricultural University;Institute of Animal Husbandry and Veterinary Medicine,Jiangri Academy of Agricultural Sciences)
出处
《畜牧兽医杂志》
2023年第5期1-6,共6页
Journal of Animal Science and Veterinary Medicine
基金
陕西省教育厅2019年度专项科学研究计划《酸败油脂及抗氧化剂对肉鸡生产性能、抗氧化体系及器官发育的影响》(19JK0052)。
关键词
余干乌骨鸡
蛋品质
脂质代谢
蛋黄胆固醇含量
血清抗氧化
Yugan silky chicken
egg quality
lipid metabolism
egg yolk cholesterol content
serum antioxidant