摘要
目的探究紫檀芪对骨质疏松大鼠牙种植体骨整合的影响以及可能的作用机制。方法采用去势法建立骨质疏松大鼠模型,造模成功后进行牙种植体植入。将牙种植体植入成功大鼠随机分为模型组、紫檀芪低(20 mg·kg^(-1))、中(40 mg·kg^(-1))、高(80 mg·kg^(-1))剂量组,每组12只;另取12只不去势大鼠作为假手术组。紫檀芪各剂量组灌胃给药,模型组与假手术组给予等体积生理盐水,1次/d,连续给药12周。采用micro-CT扫描仪进行种植区骨组织分析,亚甲基蓝-酸性品红染色观察种植区骨组织形态变化以及骨愈合情况,采用扭矩测试仪测定种植区骨组织脱位扭矩,免疫组化法检测种植区骨组织中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)表达,Western blot法检测种植区骨组织中骨形态发生蛋白2(BMP-2)、Runt相关转录因子2(Runx2)蛋白表达。结果与假手术组比较,模型组大鼠种植体与股骨结合不够紧密,结合处骨板较薄,骨小梁间隙大,且数量较少;种植区股骨骨体积分数(BV/TV)、骨小梁数量(Tb.N)和骨结合指数(OI)、种植体骨结合率与松质骨区骨量以及脱位扭矩均显著降低(P<0.05),骨小梁间隙(Tb.Sp)显著升高(P<0.05),种植区骨组织中TNF-α、IL-1β表达均显著升高(P<0.05),BMP-2、Runx2表达均显著降低(P<0.05)。与模型组比较,紫檀芪各剂量组大鼠种植体骨结合逐渐紧密,骨小梁数量逐渐增加,间隙变小,骨板增厚;大鼠种植区股骨BV/TV、Tb.N和OI、种植体骨结合率与松质骨区骨量以及脱位扭矩均显著升高(P<0.05),Tb.Sp显著降低(P<0.05),种植区骨组织中TNF-α、IL-1β表达均显著降低(P<0.05),BMP-2、Runx2表达均显著升高(P<0.05),且不同剂量组间上述指标的差异均有统计学意义(P<0.05)。结论紫檀芪可通过减轻骨质疏松引起的炎症反应,上调成骨因子BMP-2、Runx2表达,在骨质疏松状态下对牙种植体骨整合具有良好的促进作用。
Objective To explore the effect of pterostilbene on the osseointegration of dental implants in rats with osteoporosis and its possible mechanism.Methods A osteoporotic rat model was established by castration,and dental implants were implanted after successful modeling.Rats with successful dental implants were randomly divided into model group,pterostilbene low(20 mg·kg^(-1)),medium(40 mg·kg^(-1))and high(80 mg·kg^(-1))doses groups with 12 in each group.Another 12 rats without castration were taken as the sham operation group.All dose groups of pterostilbene were given by gavage,and the model group and sham operation group were given the same volume of normal saline,once a day for 12 weeks.The bone tissue analysis of the implanted area was performed using a micro-CT scanner.Methylene blueacid fuchsin staining was used to observe the morphological changes and bone healing of the implanted area.A torque tester was used to measure the bone dislocation torque at the implant site.Immunohistochemical method was used to detect the expressions of tumor necrosis factorα(TNF-α)and interleukin 1β(IL-1β)in the bone tissue of the implanted area.Western blot method was used to detect the protein expressions of bone morphogenetic protein-2(BMP-2)and runt-related transcription factor 2(Runx2)in bone tissue of implanted area.Results Compared with the sham operation group,the implants in the model group were not tightly integrated with the femur,the bone plate at the joint was thinner,the trabecular bone gap was large,and the number was small;femoral trabecular bone volume ratio(BV/TV),trabecular number(Tb.N)and osseointegrationindex(OI),implant osseointegration rate,bone mass and dislocation torque in cancellous bone area were obviously decreased(P<0.05),trabecular separation(Tb.Sp)was obviously increased(P<0.05),the expressions of TNF-αand IL-1βin the bone tissue of the implanted area were obviously increased(P<0.05),and the expressions of BMP-2 and Runx2 were obviously decreased(P<0.05).Compared with the model group,the osseointegration of the rat implants in all dose groups of pterostilbene became tighter,the number of trabecular bone gradually increased,the gap became smaller,and the bone plate thickened;femoral BV/TV,Tb.N,OI,implant osseointegration rate,bone mass and dislocation torque in cancellous bone area were obviously increased(P<0.05),Tb.Sp was obviously decreased(P<0.05),the expressions of TNF-αand IL-1βin the bone tissue of the implanted area were obviously decreased(P<0.05),and the expressions of BMP-2 and Runx2 were obviously increased(P<0.05),and there were statistically significant differences in the above indexes among different dose groups(P<0.05).Conclusion Pterostilbene can reduce the inflammatory response caused by osteoporosis,up-regulate the expressions of osteogenic factors BMP-2 and Runx2,and has a good promotion effect on the osseointegration of dental implants in the state of osteoporosis.
作者
冯亚梅
杨鹏
赵海蛟
Ya-Mei FENG;Peng YANG;Hai-Jiao ZHAO(Department of Prosthodontics,Handan Stomatological Hospital,Handan 056001,Hebei Province,China;Department of Implantology,Handan Stomatological Hospital,Handan 056001,Hebei Province,China)
出处
《药物流行病学杂志》
CAS
2023年第6期662-670,共9页
Chinese Journal of Pharmacoepidemiology
基金
河北省医学科学研究课题计划(20191817)。
关键词
紫檀芪
骨质疏松
牙种植体
骨整合
Pterostilbene
Osteoporosis
Dental implants
Osseointegration
