摘要
目的观察不同剂量黄芩苷对横纹肌溶解综合征(RM)导致急性肾损伤(AKI)大鼠的保护作用,探讨其可能的作用机制。方法选取健康SD雄性大鼠30只作为研究对象,采用随机数字表法分为阴性对照组、阳性对照组、黄芩苷低剂量干预组(低剂量组)、黄芩苷中剂量干预组(中剂量组)和黄芩苷高剂量干预组(高剂量组),每组6只。使用甘油注射法制作RM导致AKI大鼠模型,低、中、高剂量组分别于注射甘油前20 min腹腔注射不同水平的黄芩苷溶液(50、100、200 mg/kg)。采用苏木素-伊红染色观察肾组织病理学变化并同时检测血清尿素氮(BUN)、肌酐(Cr)和肌酸激酶(CK)水平。应用比色法测定肾组织中超氧化物歧化酶(SOD)及丙二醛(MDA)水平。采用实时荧光定量聚合酶链反应(RT-qPCR)检测肾脏中Klotho的基因表达水平。结果黄芩苷干预组的肾组织损伤程度与阳性对照组比较均有所减轻,炎症细胞浸润明显减少,且随着黄芩苷剂量的升高,肾组织损伤程度明显减轻。低、中、高剂量组血清BUN、Cr、CK水平均高于阴性对照组,但明显低于阳性对照组,且高剂量组<中剂量组<低剂量组,差异均有统计学意义(P<0.05)。与阳性对照组比较,黄芩苷干预组血清MDA水平明显下降,且高剂量组<中剂量组<低剂量组,差异均有统计学意义(P<0.05);血清SOD水平在黄芩苷低、中、高剂量组中明显高于阳性对照组,且高剂量组>中剂量组>低剂量组,差异均有统计学意义(P<0.05)。RT-qPCR结果显示,低、中、高剂量组Klotho基因表达量分别为(2.43±0.49)、(4.15±0.24)和(5.82±0.36),明显高于阳性对照组的(2.12±0.25),且高剂量组>中剂量组>低剂量组,差异均有统计学意义(P<0.05)。结论黄芩苷可明显改善大鼠RM导致的AKI,其机制可能与促进Klotho基因表达和减轻氧化应激相关。
Objective To observe the protective effect of baicalin at different doses on acute kidney injury(AKI)caused by rhabdomyolysis(RM),and explore the possible mechanism of action.Methods A total of 30 male Sprague-Dawley rats were selected as the study objects,which were randomly divided into negative control group,positive control group,low-dose baicalin intervention group(low dose group),medium-dose baicalin intervention group(medium dose group),and high-dose baicalin intervention group(high dose group),with 6 rats in each group.AKI rat model was induced by RM by glycerol injection.The low,medium and high dose groups were intraperitoneally injected with different levels of baicalin solution(50,100,200 mg/kg)20 min after injection of glycerin.Hematoxylin-eosin staining was used to observe the pathological changes of renal tissue,and serum urea nitrogen(BUN),creatinine(Cr)and creatine kinase(CK)levels were detected.The levels of superoxide dismutase(SOD)and malondialdehyde(MDA)in renal tissue were measured by colorimetry.The expression of Klotho gene in kidney was detected by real-time fluorescent quantitative polymerase chain reaction(RT-qPCR).Results Baicalin intervention group of renal tissue damage degree compared with positive control group are mitigated,inflammatory cells infiltration decreased significantly,and with the increase of baicalin dose,significantly reduce renal tissue damage degree.The levels of BUN,Cr and CK in the low,medium and high dose groups were higher than those in the negative control group,but significantly lower than those in the positive control group,and high dose group<medium dose group<low dose group,and the differences were statistically significant(P<0.05).Compared with the positive control group,the serum MDA level of baicalin intervention group was significantly decreased,and high dose group<middle dose group<low dose group,the differences were statistically significant(P<0.05).Serum SOD level was significantly increased in baicalin low,medium and high dose groups,and high dose group>medium dose group>low dose group,the differences were statistically significant(P<0.05).RT-qPCR results showed that the expression of Klotho gene was(2.43±0.49),(4.15±0.24)and(5.82±0.36)in low,medium and high dose respectively,which was significantly higher than that in positive control group(2.12±0.25),and high dose group>medium dose group>low dose group,and the differences were statistically significant(P<0.05).Conclusion Baicalin can significantly improve AKI induced by RM in rats,and the mechanism may be related to promoting Klotho gene expression and reducing oxidative stress.
作者
李祖攀
谷江
张永春
杨清滔
刘淼
LI Zupan;GU Jiang;ZHANG Yongchun;YANG Qingtao;LIU Miao(Department of Urology,Affiliated Hospital of Guizhou Medical University,Guiyang,Guizhou 550004,China;Department of Urology,Armed Police Corps Hospital of Guizhou Province,Guiyang,Guizhou 550005,China)
出处
《检验医学与临床》
CAS
2023年第17期2465-2468,共4页
Laboratory Medicine and Clinic
基金
贵州省贵阳市科技计划项目(20141001-45)。
