摘要
为探索芋淀粉合成机理,本研究从靖江香沙芋中克隆获得了3个芋可溶性淀粉合酶(CeSS)家族基因。3个基因CeSSI、CeSSII、CeSSIII碱基序列全长分别为1932 bp、2409 bp、3606 bp,编码形成3个亲水性蛋白质。系统进化分析结果显示,3个蛋白质CeSSI、CeSSII、CeSSIII与海枣、芦笋、药用稻等单子叶植物的亲缘关系较近。表达分析结果表明,这3个CeSS基因广泛存在于芋的叶片、叶柄、母芋和子芋中。CeSSI和CeSSII的表达水平随芋球茎的发育而显著增加,在种植150 d达到峰值,而CeSSIII在成熟后期(种植180 d)的芋球茎中表达量较高。
To explore the mechanism of starch synthesis in taro,three genes of soluble starch synthase(CeSS)family were isolated from Jingjiang Xiangsha taro.The three genes,CeSSI,CeSSII and CeSSIII,were 1932 bp,2409 bp and 3606 bp in length,respectively,and encoded three hydrophilic proteins.Phylogenetic analysis results showed that three proteins,CeSSI,CeSSII and CeSSIII,were closely related to monocotyledonous plants such as date palm,asparagus and medicinal wild rice.The results of expression analysis indicated that these three CeSS genes were widely existed in leaves,petioles,main corms and lateral cormels of taro.The expression levels of CeSSI and CeSSII increased significantly with the development of taro corms,and reached the peak at 150 d after planting,while the expression level of CeSSIII was higher in taro corms at the late maturity stage(180 d after planting).
作者
王立
殷剑美
韩晓勇
蒋璐
郭文琦
金林
张培通
WANG Li;YIN Jian-mei;HAN Xiao-yong;JIANG Lu;GUO Wen-qi;JIN Lin;ZHANG Pei-tong(Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
出处
《江苏农业学报》
CSCD
北大核心
2023年第4期939-946,共8页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金青年科学基金项目(31501776)
江苏省自然科学基金面上项目(BK20201238)
江苏省种业振兴揭榜挂帅项目[JBGS(2021)010]。
关键词
芋
可溶性淀粉合成酶
基因克隆
表达分析
Colocasia esculenta(L.)Schott
soluble starch synthase
gene cloning
expression analysis
