摘要
目的 了解鸡肉源耐甲氧西林金黄色葡萄球菌(Methicillin-resistant Staphylococcus aureus,MRSA)的全基因组特征。方法 收集7株鸡肉源MRSA菌进行全基因组测序,利用比较基因组学分析MRSA的基因特征、抗生素耐药基因(Antibiotic resistance genes, ARGs)和毒力基因携带情况,并构建基于MRSA全基因组SNP的系统发育树以分析不同菌株间的进化关系。结果 7株MRSA染色体基因组长度约为2.8 Mbp,鉴定出2 499~2 735个基因,基因组的平均GC含量为33.58%。基因功能注释结果显示测序MRSA菌株基因组中包含大量与营养物质代谢功能和环境响应相关的基因,可能为其在不同环境下的生长繁殖提供有利条件。7株鸡肉源MRSA菌的基因组中检出12~21种ARGs以及53~71种毒力基因。系统发育树显示不同ST型MRSA菌株分别处于不同的分支,而相同ST型MRSA菌株的进化距离较近,遗传进化关系较近。结论 鸡肉源MRSA菌株基因组中存在大量的ARGs和毒力基因,对人类健康造成巨大威胁,因此应持续监测并采取有效措施降低鸡肉制品中MRSA的污染水平,以确保食品安全。
Objective To understand the whole-genome characterization of methicillin-resistant Staphylococcus aureus(MRSA)from chicken.Methods The whole-genome sequence(WGS)of seven MRSA strains isolated from chicken was sequenced,and the genetic characteristics,antibiotic resistance genes(ARGs)and virulence genes was were analyzed.Meanwhile,the phylogenetic tree,based on whole-genome SNP analysis,was constructed to analyze the evolutionary rela-tionships among different MRSA strains.Results The results indicated that the full-length genomic sequence of the seven MRSA strains was about 2.8Mbp,of which 2499-2735genes were identified,and the average GC content of the genes in the seven genomes was 33.58%.The results of gene function annotation indicated that the genome of sequenced MRSA strains contains a large number of genes related to nutrient metabolism and environmental response,which may provide favorable conditions for their growth and reproduction in different environments.A total of 12-21ARGs and 53-71 virulence genes were detected in the genomes from seven MRSA strains isolated from chicken.Phylogenetic tree analysis revealed that MRSA strains with different sequence types(ST)were in different branches,whereas MRSA strains with the same ST exhibited close evolutionary distance and indicated their close genetic evolutionary relationships.Conclusion The genome of MRSA strains isolated from chicken harbors a considerable abundance of ARGs and virulence genes,po-sing agreat threat to human health.Hence,continuous surveillance and effective measures are essential to attenuate MR-SA contamination levels in poultry-derived products,thereby ensuring food safety.
作者
姜华
何晓娟
卢星辰
李晨程
李远宏
JIANG Hua;HE Xiaojuan;LU Xingchen;LI Chencheng;LI Yuanhong(School of Public Health,Xuzhou Medical College,Xuzhou 221004,Jiangsu,China)
出处
《中国病原生物学杂志》
CSCD
北大核心
2023年第10期1117-1121,1126,共6页
Journal of Pathogen Biology
基金
徐州市科技计划项目(No.KC18204)。
关键词
耐甲氧西林金黄色葡萄球菌
全基因组测序
抗生素耐药基因
毒力基因
methicillin-resistant Staphylococcus aureus
whole genome sequencing
antibiotic resistance gene
viru-lence gene
