摘要
目的探究血清25-羟维生素D[25(OH)D]和白细胞介素-22(IL-22)对急性肺炎患儿病情的诊断价值。方法选取2021年3月至2022年3月自贡市第一人民医院收治的96例急性重症肺炎患儿作为重症肺炎组,另选96例普通肺炎患儿作为普通肺炎组、96例体检健康儿童作为健康对照组。采用化学发光微粒子免疫检测法检测血清25(OH)D水平,采用酶联免疫吸附法(ELISA)检测IL-22水平;采用Pearson法分析重症肺炎患儿血清25(OH)D、IL-22水平与急慢性健康状况评分Ⅱ(APACHEⅡ评分)、序贯器官衰竭评分(SOFA评分)的相关性;采用受试者工作特征(ROC)曲线评估25(OH)D、IL-22对重症肺炎患儿危重症的诊断价值。结果重症肺炎患儿血清25(OH)D与APACHEⅡ评分及SOFA评分呈负相关(r=-0.521、-0.484,P<0.05),血清IL-22与APACHEⅡ评分及SOFA评分呈负相关(r=-0.614、-0.419,P<0.05)。ROC曲线显示,25(OH)D对危重症诊断的曲线下面积(AUC)为0.745,IL-22对危重症诊断的AUC为0.851,二者联合对危重症诊断的AUC为0.906,高于二者单独诊断(P<0.05)。结论25(OH)D与IL-22在急性重症肺炎患儿血清中低表达,二者联合用于重症肺炎患儿病情的诊断价值较高。
Objective To explore the diagnostic value of serum 25-hydroxyvitamin D[25(OH)D]and interleukin-22(IL-22)in children with acute pneumonia.Methods From March 2021 to March 2022,96 children with acute severe pneumonia admitted to Zigong First People’s Hospital were regarded as the study subjects and served as the severe pneumonia group,another 96 children with common pneumonia were served as the common pneumonia group and 96 healthy children were recruited as the healthy control group.Serum 25(OH)D level was measured by chemiluminescent particle immunoassay,and the level of IL-22 was detected by enzyme-linked immunosorbent assay(ELISA);the correlation between serum 25(OH)D,IL-22 levels and APACHEⅡscore and SOFA score in children with severe pneumonia was analyzed by Pearson method;the diagnostic value of 25(OH)D and IL-22 in critically ill children with severe pneumonia was evaluated by ROC curve.Results Serum 25(OH)D was negatively correlated with APACHEⅡscore and SOFA score in children with severe pneumonia(r=-0.521,-0.484,P<0.05),and serum IL-22 was negatively correlated with APACHEⅡscore and SOFA score(r=-0.614,-0.419,P<0.05).ROC curve showed that the area under curve(AUC)of 25(OH)D for critical disease diagnosis was 0.745,that of IL-22 for critical disease diagnosis was 0.851,and that of the combination of IL-22 for critical disease diagnosis was 0.906,which was significantly higher than that of the two diagnoses alone(P<0.05).Conclusions The expression of 25(OH)D and IL-22 is low in serum of children with acute severe pneumonia.The combination of 25(OH)D and IL-22 is of high value in the diagnosis of severe pneumonia.
作者
陈喻
温友利
余建洪
张肃川
CHEN Yu;WEN Youli;YU Jianhong;ZHANG Suchuan(Department of Laboratory,Zigong First People's Hospital,Zigong 643000,China;Department of Respiratory,Zigong First People's Hospital,Zigong 643000,China)
出处
《安徽医学》
2023年第9期1051-1054,共4页
Anhui Medical Journal
基金
自贡市卫生健康科研课题(编号:20zd001)。
