摘要
目的探讨三七总皂苷(PNS)对重症急性胰腺炎(SAP)病理、炎症、感染、肝功能和心肌酶谱的影响。方法SD大鼠随机分为Sham(假手术组)、SAP(模型组)、L-PNS、H-PNS和SP600125组;L-PNS、H-PNS和SP600125组分别在造模前腹腔注射低剂量PNS,高剂量PNS和阳性药物SP600125。造模后3、6、12和24 h,分别取胰腺和肺组织进行苏木精-伊红染色,取肺组织进行免疫组化染色和MPO,IL-1β和TNF-α水平的检测,取血清进行CRP,PCT,IL-6,α-AMS,ALT,CK-MB和LDH水平的检测。结果SAP模型构建成功,且相较于各时间点的SAP组,对应各时间点的L-PNS,H-PNS和SP600125组中胰腺组织和肺组织病理评分、肺组织中ICAM-1阳性细胞比例及MPO,IL-1β和TNF-α水平、血清中CRP,PCT,IL-6,α-AMS,ALT,CK-MB和LDH水平均显著降低(P<0.05)。这些指标在各时间点的H-PNS和SP600125组中无显著性差异(P>0.05)。结论PNS有效缓解胰腺损伤、肺损伤、肝功能和心肌酶谱异常。
Objective To investigate the effects of Panax Notoginseng Saponin(PNS)on the pathology,inflammation,infection,liver function and myocardial enzyme of severe acute pancreatitis(SAP).Method SD rats were randomly divided into Sham(sham-operated group),SAP(model group),L-PNS,H-PNS and SP600125 groups;L-PNS,H-PNS and SP600125 groups were injected intraperitoneally with low-dose PNS,high-dose PNS and positive drug SP600125 before molding.At 3h,6h,12h and 24h after molding,pancreas and lung tissues were taken for hematoxylin-eosin staining,lung tissues were taken for immunohistochemical staining and measurement of MPO,IL-1β and TNF-α levels,and serum was taken for measurement of CRP,PCT,IL-6,α-AMS,ALT,CK-MB and LDH levels.Results The SAP model was successfully constructed.Compared to the SAP group at each time point,the pancreatic tissue and lung histopathological scores,the proportion of ICAM-1 positive cells and the levels of MPO,IL-1β and TNF-α in lung tissue,as well as the levels of CRP,PCT,IL-6,α-AMS,ALT,CK-MB and LDH in serum were significantly reduced(P<0.05).These indicators were not significantly different between the H-PNS and SP600125 groups at each time point(P>0.05).Conclusion PNS effectively alleviates pancreatic and lung injury,as well as abnormal liver function and myocardial enzyme,providing potential application to clinical treatment strategies for SAP.
作者
张帆
万红芬
胡宜涛
丁琦
李泗云
刘界宇
张昱
ZHANG Fan;WAN Hong-fen;HU Yi-tao;DING Qi;LI Si-yun;LIU Jie-yu;ZHANG Yu(Department of Gastroenterology,Yunnan Third People's Hospital,Kunming 650011,China;Department of Gastroenterology,Yunnan First People's Hospital,Kunming 650032,China)
出处
《现代消化及介入诊疗》
2023年第6期714-718,共5页
Modern Interventional Diagnosis and Treatment in Gastroenterology
基金
云南省“兴滇英才支持计划”名医(XDYC-MY-2022-0007)。
关键词
重症急性胰腺炎
三七总皂苷
多器官损伤
炎性因子
Severe acute pancreatitis
Panax Notoginseng Saponins
Multiple organ dysfunction
Inflammatory factor
