摘要
芽孢杆菌具有促进植物生长并提高植物抗病害能力,是农业生产中的重要微生物资源.基于形态学、生理生化特征及16S rRNA系统发育关系对菌株A-1进行分类鉴定;以油菜作为实验对象,测定盐胁迫下接种菌株A-1对油菜的促生长能力;通过拮抗试验,测定菌株A-1对油菜黑胫病菌丝的抑制效果;采用全基因组测序方法,探究菌株A-1的生物防治机理.结果表明,该菌株属于Bacillus swezeyi;在盐胁迫下,接种A-1菌株的油菜根长、株高和鲜重较对照组分别增加了42.35%、27.24%和105.61%;菌株A-1显著抑制油菜黑胫病菌丝生长并引起菌丝细胞溶解;菌株A-1全基因组大小为4591993 bp,G+C含量为44.39%,共编码4948个基因,含有85个tRNA基因,24个rRNA基因和38个sRNA基因,存在编码水解病原真菌细胞壁的功能酶基因和IAA合成相关基因,以及7个参与芽孢杆菌属抗菌物质合成的基因簇.本研究工作将为B.swezeyi菌肥和生防菌剂的开发奠定基础.
Bacillus is an important microbial resource in agricultural production,which can promote plant growth and improve plant disease resistance.Based on morphological,physiological and biochemical characteristics and 16S rRNA phylogenetic relationship,strain A-1 was classified and identified.Brassica na pus was used as the experimental object to determine the growth-promoting ability of inoculated strain A-1 under salt stress.The inhibitory effect of strain A-1 on the mycelium of Leptosphaeria biglobosa was determined by antagonistic test.The biological control mechanism of strain A-1 was explored by whole genome sequencing.The results showed that strain A-1 was classified as Bacillus swezeyi.Under salt stress,the root length,plant height and fresh weight of rape inoculated with A-1 strain increased by 42.35%,27.24%and 105.61%,respectively,compared with the control group.Strain A-1 significantly inhibited the mycelial growth of Leptosphaeria biglobosa and caused mycelial cell lysis.The whole genome size of strain A-1 was 4591993 bp,and the G+C content was 44.39%.A total of 4948 genes were encoded,including 85 tRNA genes,24 rRNA genes and 38 sRNA genes.There are genes encoding functional enzymes that hydrolyze the cell wall of pathogenic fungi and genes related to IAA synthesis,as well as seven gene clusters involved in the synthesis of antimicrobial substances in Bacillus.This study will lay the foundation for the development of B.swezeyi bacterial fertilizer and biocontrol agents.
作者
刘开辉
刘月
陈妮
丁小维
张智维
刘婉婷
LIU Kai-hui;LIU Yue;CHEN Ni;DING Xiao-wei;ZHANG Zhi-wei;LIU Wan-ting(School of Food Science and Engineering,Shaanxi University of Science&Technology,Xi′an 710021,China)
出处
《陕西科技大学学报》
北大核心
2023年第5期64-69,86,共7页
Journal of Shaanxi University of Science & Technology
基金
国家自然科学基金项目(31970120)
陕西省科技厅社会发展科技计划项目(2023-YBSF-325)。
关键词
芽孢杆菌
全基因组测序
生防菌剂
Bacillus
whole genome sequencing
biocontrol agents
