摘要
湖光岩玛珥湖(Huguangyan Maar Lake)地理环境特殊,对于挖掘特殊生境耐热真菌资源具有较大潜力。对1株来源于湖光岩玛珥湖的耐热真菌HS1-1进行了形态观察,分子鉴定和生理特性、产酶能力、抗菌活性的测定。结果表明,该真菌为烟曲霉(Aspergillus fumigatus),菌株在15~50℃和pH3~12均能生长,最适生长条件是温度40℃,盐浓度1%~2%,pH6~7;经4种产酶培养基筛选确定菌株对可溶性淀粉、乳糖、羧甲基纤维素有较强的利用能力,对5种指示菌有一定抑制效果。说明真菌HS1-1具有较强耐热特性、酸碱耐受性和一定的抗菌活性。本研究对深入了解湖光岩玛珥湖耐热真菌并对其进一步开发利用提供理论基础。
Huguangyan Maar Lake is endowed with a special geographical environment and has a great potential for exploring thermotolerant fungal resources in special habitats.A thermotolerant fungus HS1-1 was collected from Huguangyan Maar Lake,observed morphologically and identified molecularly,and its physiological properties,enzyme production capacity and antibacterial activity were determined.The results showed that the strain of this fungus is identified as Aspergillus fumigatus.This fungus can grow at the temperatures of 15-50℃and pH3-pH12,and has an optimum growth at the temperature of 40℃,a salt concentration of 1%-2%,and pH6-pH7.The fungus has a strong ability to utilize soluble starch,lactose and carboxymethyl cellulose,and has a certain inhibitory effect on five indicator bacteria,as determined by screening on four enzyme-producing media.This indicates that the fungus HS1-1 is high in thermotolerance and acid and base tolerance with some antibacterial activities.This study provides a theoretical basis for an indepth understanding of the thermotolerant fungi of Huguangyan Marr Lake and their further development and utilization.
作者
黄晓欣
谈嘉莉
杨永星
顾哲铭
李雪珽
雷晓凌
HUANG Xiaoxin;TAN Jiali;YANG Yongxing;GU Zheming;LI Xueting;LEI Xiaoling(College of Food Science and Technology/Guangdong Key Laboratory of Aquatic Product Processing and Safety/Guangdong Engineering and Technology Research Center for Marine Food/Guangdong Engineering Laboratory for Marine Biological Products/Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution,Guangdong Ocean University,Zhanjiang,Guangdong 524088;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian,Liaoning 116034,China)
出处
《热带生物学报》
2023年第5期552-559,共8页
Journal of Tropical Biology
基金
深圳市科创委基础研究面上项目(JCYJ20190813105005619)
广东省普通高校重点领域专项(生物医药与健康)(2021ZDZX2064)
广东省科技专项资金-基础与应用基础研究专题(2021A50114)。
关键词
耐热真菌
分子鉴定
生理生长
活性研究
湖光岩
thermotolerant fungi
molecular identification
physiological growth
activity research
Huguangyan
