摘要
为了获得高抑菌性能的乳酸菌,以植物乳杆菌DY6为出发菌株,首先对其进行常压室温等离子体(atmospheric room temperature plasma,ARTP)和亚硝基胍(nitrosoguanidine,NTG)的复合诱变,获得了抑菌活性较强的诱变菌株AN-55、AN-58及AN-68,其抑菌活性分别提高了19.83%、21.91%和18.40%。随后,经遗传稳定性实验发现菌株AN-55的抑菌性能更加稳定,8次传代后其抑菌活性较野生型菌株仍能提高20.51%。与此同时,菌株AN-55的基因组重测序结果表明,plnK、cps4G、pts9D等基因序列的改变可能对提高菌株抑菌活性有重要影响,为植物乳杆菌在天然防腐剂和抗生素替代物的应用奠定了理论基础。
In order to obtain lactic acid bacteria with high bacteriostatic property,Lactobacillus plantarum DY6 was selected as the starting strain in this study,which was mutagenized by a compound mutagenesis approach combining atmospheric room temperature plasma(ARTP)and nitrosoguanidine(NTG).The mutant strains AN-55,AN-58 and AN-68 with strong antibacterial activity were obtained,whose antibacterial activity increased by 19.83%,21.91%and 18.40%,respectively.Subsequently,it was found that the bacteriostatic performance of strain AN-55 was more stable through the genetic stability test,and the antibacterial activity still increased by 20.51%even after 8 passages compared to the wild-type strain.At the same time,genome resequencing of strain AN-55 revealed that the changes of plnK,cps4G and pts9D gene sequences may have an important effect on improving the antibacterial activity of the strain,laying a theoretical foundation for the application of Lactobacillus plantarum as natural preservatives and antibiotic substitutes.
作者
毛银
卢艳波
杨小雁
马忠玛
刘颖颖
鲍青青
邓禹
MAO Yin;LU Yanbo;YANG Xiaoyan;MA Zhongma;LIU Yingying;BAO Qingqing;DENG Yu(National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing,Jiangnan University,Wuxi 214122,China;Shandong Key Laboratory of Oil and Oil Deep Processing Technology,Shandong Bohai Industrial Group Company Ltd.,Binzhou 256500,China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2023年第9期20-27,共8页
Journal of Food Science and Biotechnology
基金
山东省重点研发计划项目(2022CXPT042)
国家自然科学基金项目(21877053)
广西壮族自治区重点研发计划项目(桂科AB22035063)。
关键词
植物乳杆菌
诱变
抑菌活性
基因组重测序
Lactobacillus plantarum
mutation
antibacterial activity
genome resequencing
