摘要
[目的]铝胁迫是限制植物生长的重要非生物胁迫因子,严重制约我国南方酸性土壤森林生产力水平。为探明酸铝环境下马尾松幼苗生长变异规律,筛选出适应酸性环境耐铝毒家系和敏感性家系,为马尾松耐铝胁迫遗传改良提供依据。[方法]以55个马尾松二代优良家系(201~255)幼苗为材料,采用沙培方式研究酸性环境(pH=4.0)不同铝浓度(0、0.1、0.3、0.6、1.2、2.4 mmol·L^(−1))马尾松苗生长和根系形态变化规律,并通过主成分分析和聚类分析对马尾松耐铝特性进行分等定级,筛选和鉴定马尾松幼苗耐铝优良家系。[结果]苗高、地径、高径比、生物量(地上生物量、根生物量、总生物量、根冠比)、根系形态(总根长、根表面积、根体积)在家系间和铝浓度处理间均存在显著差异(p<0.05)。随铝浓度增加马尾松幼苗地径、苗高、生物量和根系形态指标均呈现先升后降趋势,在0.6 mmol·L^(−1)铝浓度下最大,显著高于0、2.4 mmol·L^(−1)处理,而与0.3 mmol·L^(−1)处理无显著差异,其中根生物量、总根长、根表面积和根体积的变异系数较大,平均为30.79%,属中等变异,根系可作为马尾松幼苗适应酸铝环境敏感性器官。用上述10个指标计算家系间耐铝特性综合评价值(CEV)和系统聚类,筛选出强度耐铝型家系3个(占5.45%),中度耐铝型家系11个(占20%),一般耐铝型家系37个(占67.28%),铝敏感型家系4个(占7.27%)。[结论]适宜铝浓度能够促进马尾松生长,尤其是0.3~0.6 mmol·L^(−1)铝浓度下促进效果佳,筛选得到耐铝型和敏感型家系对马尾松耐铝性状改良具有重要意义。
[Objective]Acid-aluminum stress is an important abiotic stress factor limiting plant growth,which seriously restricts the productivity of acid soil forests in southern China.In order to explore the growth variation law under acid aluminum environment of Pinus massoniana seedlings,sselect aluminum-tolerant families and sensitive families adapted to aluminum stress,and provide basis for genetic improvement of Pinus massoniana under aluminum stress.[Method]The seedlings of 55 second-generation superior families(201-255)were used to study the growth and root morphological changes under 0,0.1,0.3,0.6,1.2 and 2.4 mmol·L^(−1)aluminum concentration in acidic environment by sand culture of P.massoniana.The aluminum tolerance characteristics were graded by principal component analysis and cluster analysis.The excellent aluminum tolerance,the superior families of the seedlings were sselected and identified.[Results]There were significant differences in seedling height,ground diameter,height-diameter ratio,biomass(aboveground biomass,root biomass,total biomass,root-shoot ratio),root morphology(total root length,root surface area,root volume)between families and aluminum concentration treatments(p<0.05).The ground diameter,seedling height,biomass and root morphological indexes increased first and then decreased with the increase of aluminum concentration of the seedlings,which was the highest at 0.6 mmol·L^(−1)aluminum concentration,significantly higher than that of 0 and 2.4 mmol·L^(−1)treatment.There were no significant differences with 0.3 mmol·L^(−1)treatment.The coefficient of variation of root biomass and root morphology was large,with an average of 30.79%.Using the above 10 indicators to calculate the comprehensive evaluation value(CEV)and analyze for systematic clustering of aluminum resistance characteristics among families,3 families with strong aluminum resistance(accounting for 5.45%)and 11 families with moderate aluminum resistance(accounting for 20%)were selected.37 families(67.28%)with general aluminum tolerance,and 4 families(7.27%)with aluminum sensitivity were evaluated.[Conclusion]Suitable aluminum concentration can promote the growth,especially at the concentration of 0.3-0.6 mmol·L^(−1)aluminum.Selecting aluminum-tolerant and sensitive families is of great significance for the improvement of aluminum tolerance of P.massoniana.
作者
李鹏
谭健晖
杨章旗
罗群凤
凌金燕
陈虎
陈钊铭
贾婕
LI Peng;TAN Jian-hui;YANG Zhang-qi;LUO Qun-feng;LING Jin-yan;CHEN Hu;CHEN Zhao-ming;JIA Jie(Guangxi Academy of Forestry,National Forestry and Grass Administration,Masson Pine Engineering Technology Research Center,Guangxi Masson Pine Engineering Technology Research Center,Guangxi Key Laboratory of Fine Timber Forest Resources Cultivation,Nanning 530002,China;Collage of Life Science Guangxi Normal University,Guilin Guangxi 541006,China)
出处
《林业科学研究》
CSCD
北大核心
2023年第5期121-130,共10页
Forest Research
基金
广西优良用材林资源培育重点实验室开放课题(2020-A-02-03)
广西自然科学基金(2019GXNSFDA245033)
广西科技基地和人才专项(桂AD19254004)
八桂学者项目(2019A026)资助。
关键词
马尾松
二代家系
铝胁迫
耐铝特性
主成分分析
Pinus massoniana
second generation family
aluminum stress
aluminum resistance characteristics
principal component analysis
