摘要
目的:现场快速筛查鸡蛋中恩诺沙星。方法:按《食品安全国家标准食品中41种兽药最大残留限量》要求,依据国家市场监督管理总局2023年1月发布的《关于规范食品快速检测使用的意见》,制备3个浓度水平的恩诺沙星加标样本。50份空白样本和50份标准限量要求0.5倍浓度水平(5μg/kg)的加标样本用以评价快检方法的假阳性率;50份标准限量要求1倍浓度水平(10μg/kg)的加标样本用以评价快检方法的假阴性率。50份实际鸡蛋样本用量子点微球免疫层析试纸条与HPLC-MS/MS方法分别进行检测。结果:量子点微球免疫层析试纸条可以满足国家限量要求,假阴性率为0%,假阳性率为0%。实际样本检测时,量子点微球免疫层析试纸条与HPLC-MS/MS方法的结果符合率高。结论:借助于便携式读取仪,量子点微球免疫层析法可获得数字化的准确结果,适合鸡蛋中风险因子的大批量样本现场快速筛查。
Objective:This study aimed to realize rapid on-site screening of enrofloxacin in eggs.Methods:According to the requirements of the“Maximum residue limit of 41 veterinary drugs in the national food safety standard”and the“Opinions on regulating the use of rapid food testing”issued by the State Market Supervision and Administration in January 2023,the spiked samples at three concentration levels of enrofloxacin were prepared.Fifty blank samples and fifty spiked samples at a concentration level of 0.5 time the standard limit requirement(5μg/kg)were used to evaluate the false positive rate of the rapid detection method.Fifty spiked samples at a concentration level of 1 time the standard limit requirement(10μg/kg)were used to evaluate the false negative rate of the rapid detection method.Fifty real samples of eggs were detected with immunochromatographic test strips based on quantum dot microsphere and HPLC-MS/MS,respectively.Results:The immunochromatographic test strips based on quantum dot microsphere can meet the national limit requirements,with false negative rate of 0%and false positive rate of 0%.For the detection with real samples,the results of the immunochromatographic test strips based on quantum dot microsphere have a high coincidence rate with that of HPLC-MS/MS.Conclusion:With the help of a portable reader,quantum-dot microsphere immunochromatography can obtain digital and accurate results,which is suitable for rapid on-site screening of large samples of risk factors in eggs.
作者
张富生
王宇宸
卢礼生
邓家军
陈俊松
杨琳芬
郭静
吴仁伟
赖卫华
ZHANG Fusheng;WANG Yuchen;LU Lisheng;DENG Jiajun;CHEN Junsong;YANG Linfen;GUO Jing;WU Renwei;LAI Weihua(Jiangxi Agricultural Product Quality Safety and Inspection Center,Nanchang,Jiangxi 330077,China;State Key Laboratory of Food Science and Resources,Nanchang University,Nanchang,Jiangxi 330047,China;Jiujiang Agricultural Product Quality Safety Center,Jiujiang,Jiangxi 332000,China)
出处
《食品与机械》
CSCD
北大核心
2023年第9期32-37,70,共7页
Food and Machinery
基金
国家重点研发计划(编号:2019YFC1605502)
江西国家科技奖后备培育项目(编号:20223AEI91011)。
关键词
量子点微球免疫层析试纸条
恩诺沙星
鸡蛋
性能评价
immunochromatographic test strips based on quantum dot microsphere
enrofloxacin
eggs
performance evaluation
