摘要
目的观察针刀对膝骨关节炎(KOA)兔软骨NLRP3/Caspase-1通路的调控作用及软骨细胞焦亡的影响.方法选用24只新西兰兔雄兔,年龄均为6个月.使用随机数字表法将其分为3组,分别为空白组、模型组、针刀组,每组各8只.造模方法采用改良Videman法进行固定,造模时间为6周,空白组不予固定.针刀组选取经筋病灶点行针刀松解治疗,每周1次,共治疗3次;空白组和模型组正常饲养,行同样抓捕行为.采用HE染色观察软骨形态学改变;并检测软骨Caspase-1、NLRP3、ASC、IL-1βmRNA和蛋白的表达.结果空白组软骨细胞均匀地分布在软骨基质中,表面相对光滑,层次结构较为清晰,潮线清晰完整.模型组软骨细胞数量较少,且分布状态较不均匀,表层出现缺损,潮线紊乱.与模型组相比,针刀组软骨表面较光滑,且软骨细胞分布较均匀,排列较规整,潮线较完整.与空白组比较,模型组软骨组织Caspase-1、NLRP3、IL-1βmRNA表达显著上调(P<0.05);与模型组比较,针刀组软骨组织Caspase-1、NLRP3、IL-1βmRNA表达显著下调(P<0.05).与空白组比较,模型组中兔软骨组织Caspase-1、NLRP3、IL-1βmRNA和Caspase-1、NLRP3、IL-1β、ASC蛋白表达水平显著升高(P<0.05).针刀干预后兔软骨细胞排列较模型组改善,软骨细胞数量增多,Caspase-1、NLRP3、IL-1βmRNA和Caspase-1、NLRP3、IL-1β、ASC蛋白表达水平显著下调(P<0.05).结论基于经筋理论运用针刀治疗KOA,延缓软骨病变,从而改善症状,其作用机制可能是通过干预软骨细胞焦亡,从而降低关节内炎症介质来实现的.
Objective To observe the effects of acupotomy on the regulation of the NLRP3/Caspase-1 pathway and chondrocyte pyroptosis in rabbit cartilage with knee osteoarthritis(KOA).Methods Twenty-four male New Zealand rabbits,all aged six months,were used.They were divided into three groups using the random number table method:blank group,model group,and acupotomy group.The modeling method was fixed by the modified Videman method for six weeks,and the blank group was not fixed.In the acupotomy group,the transversal tendon lesion was treated with needle knife,once a week,for a total of three times;the blank group and the model group were not treated.The morphological changes of cartilage were observed by HE staining,and the expression of cartilage Caspase-1,NLRP3,ASC and IL-1βin the cells were detected.Results The chondrocytes in the blank group were uniformly distributed in the cartilage matrix,with relatively smooth surface,clearer hierarchical structure and clear and complete tide lines.In the model group,the number of chondrocytes was smaller,and the distribution was uneven,with defects in the surface layer and disturbed tide lines.Compared with the model group,the cartilage surface of the needle knife group was smoother,and the chondrocytes were more evenly distributed and arranged,and the tide lines were more complete.Compared with the blank group,cartilage tissue Caspase-1,NLRP3,IL-1βmRNA expression was significantly up-regulated in the model group(P<0.05);compared with the model group,cartilage tissue Caspase-1,NLRP3,IL-1βmRNA expression was significantly down-regulated in the needle-knife group(P<0.05).Compared with the blank group,the expression levels of Caspase-1,NLRP3,IL-1βmRNA and Caspase-1,NLRP3,IL-1β,ASC protein in rabbit cartilage tissue were significantly increased in the model group(P<0.05).Chondrocyte alignment was improved in rabbits after acupotomy intervention compared with the model group,the number of chondrocytes was increased,and the expression levels of Caspase-1,NLRP3,IL-1βmRNA and Caspase-1,NLRP3,IL-1β,ASC protein were significantly downregulated(P<0.05).Conclusion Treating KOA with acupotomy based on the theory of meridian theory can delay cartilage lesions and improve symptoms.The mechanism of action may be reducing the intra-articular inflammatory mediators by intervening chondrocyte pyroptosis.
作者
张良志
刘洪
谢梓毅
李阳阳
余文英
刘晶
修忠标
ZHANG Liangzhi;LIU Hong;XIE Ziyi;LI Yangyang;YU Wenying;LIU Jin;XIU Zongbiao(Second Department of Orthopedics-traumatology,The People's Hospital Affiliated of Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350004,China)
出处
《福建医药杂志》
CAS
2023年第5期87-90,F0003,共5页
Fujian Medical Journal
基金
福建省自然科学基金面上项目(2022J01354)
福建中医药大学中医骨伤及运动康复教育部重点实验室开放课题(ZD2020-1-4)。
