摘要
为探究百里香属三种百里香植物挥发油的化学成分及其抑菌和抗癌作用,采用水蒸气蒸馏法分别提取柠檬百里香、百里香和法国百里香中的挥发油,并对其化学成分进行分析,共检测出50种化合物,其中百里香酚含量最高37.61%,柠檬醛11.44%、顺式香叶醇10.25%、石竹素5.74%、异百里酚甲醚12.45%。采用滤纸片抑菌法和平板涂布法检测三种挥发油的抑菌活性,确定柠檬百里香挥发油的抑菌效果最好,最小抑菌/杀菌浓度强弱依次为大肠杆菌(Escherichia coli)>燕麦镰刀菌(Fusarium avenaceum)>金黄色葡萄球菌(Staphylococcus aureus)。CCK-8法测定三种百里香挥发油对肝癌细胞HepG2的毒性,结果表明柠檬百里香挥发油对肝癌细胞的抑制作用最强,其IC_(50)值为18.32μg/mL。该研究结果可为百里香挥发油的开发利用提供理论依据。
In order to characterize the chemical components in three thyme plants from thyme genus and evaluate their bacteriostatic activity and cytotoxicity on human cancer cells,the volatile oil from T.citriodorus,T.mongolicus,and T.vulgaris was extracted by steam distillation and their chemical components were analyzed.A total of 50 compounds were detected,of which the highest thymol content was 37.61%,citral was 11.44%,cis-geraniol was 10.25%,caryophyllene oxide was 5.74%,and thymol methyl ether was 12.45%.The antibacterial activity from these three types volatile oils was evaluted by filter paper method and plate coating method and volatile oil of T.citriodorus was determined as the best for its antibacterial effect compared with others.The minimum bacteriostatic concentration against three strains of bacteria was in the order of Escherichia coli>Fusarium avenaceum>Staphylococcus aureus.The CCK-8 method was used to determine the cytotoxicity of three thyme volatile oils on HepG2 liver cancer cells.The results showed that volatile oil from T.citriodorus had the strongest inhibitory effect on liver cancer cells with an IC_(50)value of 18.32μg/mL.The results from this research can provide a theoretical basis for the development and utilization of thyme volatile oil.
作者
韩峰
孙耀文
马迎梅
郑丽娜
刘承昊
赵博扬
HAN Feng;SUN Yao-wen;MA Ying-mei;ZHENG Li-na;LIU Cheng-hao;ZHAO Bo-yang(College of Grassland Resources and Environment,Inner Mongolia Agricultural University;College of Desert Control Science and Engineering,Inner Mongolia Agricultural University;Mengcao Ecological Environment(Group)Co.,Ltd.of Inner Mongolia,Hohhot 010000,China)
出处
《天然产物研究与开发》
CAS
CSCD
2023年第10期1708-1715,共8页
Natural Product Research and Development
基金
内蒙古自治区自然科学基金-博士基金(2021BS03037,2022MS03056)。
关键词
百里香挥发油
成分分析
抑菌活性
细胞毒性
thyme essential oil
component analysis
bacteriostatic activity
cytotoxicity
