摘要
针对目前脑胶质瘤诊断治疗中靶向性差、治疗效率低等问题,设计合成以气囊蛋白(Gas vesicles,GV)为载体,透明质酸(Hyaluronic acid,HA)靶向负载阿霉素(Doxorubicin,DOX)的纳米药物递送系统。利用EDC/NHS脱水缩合反应,制备GV@hDOX载药系统,观察其对小鼠脑胶质瘤的杀伤作用以及超声成像效果。首先,通过化学反应将HA-DOX接枝在GV的表面,得到具有靶向作用的GV@hDOX;其次,通过透射电子显微镜和荧光光谱仪等仪器对GV和GV@hDOX的形貌结构进行表征;最后,对其体外稳定性、靶向肿瘤细胞吞噬与杀伤能力进行评价。透射电子显微镜和荧光光谱图表明GV@hDOX的成功合成,负载量为66.7μg/OD_(500);与GV相比,GV@hDOX具有更高的超声成像能力;与游离DOX相比,GV@hDOX显著提高了小鼠脑胶质瘤GL261细胞对DOX的吞噬效率。GV@hDOX优异的超声成像和药物递送效率为脑胶质瘤的靶向载药系统的构建提供了新的思路,为进一步实现脑胶质瘤的高效治疗奠定了基础。
The purpose of this study is to design and synthesize a new nanodrug delivery system overcoming bad targeting and low efficiency in the treatment of glioblastoma.Gas vesicles(GV)were used as carriers,hyaluronic acid(HA)served as targeting molecules,and doxorubicin(DOX)acted as the payload.The GV@hDOX drug delivery system was prepared using EDC/NHS dehydration condensation reaction,and its killing effect on mouse glioblastoma and ultrasound imaging were observed.Firstly,HA-DOX was grafted onto the surface of GV through a chemical reaction to obtain targeted GV@hDOX.Secondly,the morphology and structure of GV and GV@hDOX were characterized by transmission electron microscopy and fluorescence spectrophotometer.Finally,the in vitro stability,targeting tumor cell phagocytosis and killing ability were evaluated.GV@hDOX was successfully synthesized with a loading capacity of 66.7μg/OD_(500).Compared with GV,GV@hDOX had higher ultrasound imaging ability,and it significantly increased the phagocytic efficiency of DOX by mouse glioblastoma GL261 cells compared with free DOX.Therefore,GV@hDOX with excellent ultrasound imaging and drug delivery efficiency provides a new approach for the treatment of glioblastoma,and lays a foundation for further research in the future.
作者
崔雨童
韩佳铌
唐燕琼
马香
李宏
李娟娟
刘柱
CUI Yu-tong;HAN Jia-ni;TANG Yan-qiong;MA Xiang;LI Hong;LI Juan-juan;LIU Zhu(School of Life Sciences,Hainan University,Haikou 570208,China)
出处
《化学试剂》
CAS
北大核心
2023年第11期25-31,共7页
Chemical Reagents
基金
国家自然科学基金项目(32260020,32260028)
武汉光电国家研究中心开放基金项目(2020WNLOKF019)。
关键词
气囊蛋白
脑胶质瘤
阿霉素
载药系统
gas vesicle
glioma cells
doxorubicin
drug delivery system
