摘要
互花米草(Spartinaalterniflora)是目前中国海岸带湿地分布面积最广的入侵植被,其较强的入侵及扩散能力与其高盐度抗性密不可分。为阐明其盐度胁迫响应及抗性机制,通过对3~5叶期S.alterniflora幼苗进行10、24和32盐度条件处理,并采用Illumina高通量测序技术进行转录组测序分析:共获得121760个基因(Unigene)以及1190个差异表达基因(Differentially Expressed Genes,DEGs),挖掘得到如脯氨酸代谢相关基因(Sporobolusalterniflorusdelta1-pyrroline-5-carboxylatesynthetase2,SaP5CS2)、稳定Na+/K+比例的阳离子转运体基因(Cation transporter,HKT7)、钠/氢交换器基因(Sodium hydrogen exchanger 2,NHX2)、抗K+外流基因(K+effluxantiporter5)等与互花米草耐盐高度相关的Unigene;GO(Gene Ontology)功能注释结果表明,DEGs主要与催化活性、转运活性、细胞过程、新陈代谢过程、细胞组分等相关;KEGG(Kyoto Encyclopedia of Genes and Genomes)富集结果显示,DEGs主要富集在氰氨酸代谢通路、氧化磷酸化代谢通路、光合作用-天线蛋白通路等通路中;功能-基因互作网络分析显示,显著富集到的苯丙素代谢途径、氧化磷酸化途径(LG组与CG组),氮(N)代谢、光合作用-天线蛋白、ECM-受体相互作用、P13K-Akt信号通路、氧化磷酸化等途径(HG与CG组),氰基氨酸代谢、硫代葡萄糖苷生物合成、油菜素类固醇生物合成、角质、木栓和蜡生物合成(HG组与LG组)等途径都与互花米草抗逆性有关。本研究通过挖掘互花米草的抗盐关键基因及代谢通路,提供了其生物入侵和扩散分子机制的基础信息,为互花米草等入侵物种的防控技术方向提供了重要参考,并具有重要的经济及生态学意义。
Spartina alterniflora is the most widely distributed invasive vegetation in coastal wetlands in China.Its strong invasion and diffusion ability is inseparable from its high salinity resistance.In order to clarify its response to salinity stress and resistance mechanism,this study carried out a series of experiments on S.alterniflora with 3-5 seedings treated at 10,24,and 32 salinity conditions,and transcriptome sequencing analysis was performed using Illumina high-throughput sequencing technology.A total of 121760 unigenes and 1190 differentially expressed genes(DEGs)were obtained Genes highly related to salt tolerance of S.alterniflora,such as proline metabolism related genes(Sporobolus alterniflorus delta 1-pyrroline-5-carboxylate synthetase 2,SaP5CS2),cation transporters with stable Na+/K+ratio(cation transporter HKT7),sodium/hydrogen exchangers 2(NHX2),and resistance to k+efflux 5,were mined.Go functional annotation results showed that DEGs were mainly related to catalytic activity,transport activity,cellular processes,metabolic processes and cellular components,etc.KEGG enrichment results showed that DEGs were mainly enriched in pathways such as cyanoamino acid metabolism pathway,oxidative phosphorylation metabolism pathway and photosynthesis antenna protein pathway,etc.Function gene interaction network analysis showed that the significantly enriched phenylpropanoid metabolism pathway,oxidative phosphorylation pathway(LG vs CG Group),nitrogen metabolism,photosynthesis antenna protein,ECM receptor interaction,P13K-Akt signaling pathway,oxidative phosphorylation and other pathways(Hg vs CG Group),cyanoamino acide metabolism,glucosinolate biosynthesis,brassinosteroid biosynthesis,cutin pathways such as cork and wax biosynthesis(HG vs LG Group)were all related to the stress resistance of S.alterniflora.Therefore,this study provides basic information on the molecular mechanism of biological invasion and proliferation by mining the salt resistance key genes and metabolic pathways of S.alterniflora,and provides important references for the direction of prevention and control technology for invasive species such as S.alterniflora,and has important economic and ecological significance.
作者
王鹏娜
何英英
李文君
赵阳
曲长凤
缪锦来
WANG Pengna;HE Yingying;LI Wenjun;ZHAO Yang;QU Changfeng;MIAO Jinlai(First Institute of Oceanography,MNR,Qingdao 266061,China;Qingdao Academy of Agricultural Science,Qingdao 266199,China)
出处
《海洋科学进展》
CAS
CSCD
北大核心
2023年第4期721-736,共16页
Advances in Marine Science
基金
中央级公益性科研院所基本科研业务费专项资金资助项目(2022Q10)
广西科技基地和人才专项(AA21196002)
国家自然科学基金项目(32000074和42176130)
山东省自然科学基金项目(ZR2021MD044)
泰山产业领军人才项目(tscy20190308)
山东省重点研发计划项目(2021TZXD008)。
关键词
互花米草
盐度胁迫
转录组学
差异表达基因
Spartina alternifolia
salinity stress
transcriptome
differential expression of gene Received:June 11,2023
