摘要
目的自制一种负压血培养瓶,提升临床病原微生物诊断检验功效。方法自制的负压血培养瓶采用100 mL玻璃输液瓶,由瓶体和瓶盖构成,瓶盖密封的瓶体内呈负压状态,密封瓶体内温度为-10~65℃,湿度为小于或等于85%,压力为-50~-30 kPa,在瓶口处设有包裹瓶口的玻璃纸,玻璃纸外面包裹纱布,负压状态的瓶内装有30 mL的血培养基。血培养基配方参照《全国临床检验操作规程》第4版进行调试、消毒、负压制备等环节,进行临床性能验证实验。结果自制负压血培养瓶在所有菌株最低浓度级别时均培养出阳性,阳性符合率为100%。自制负压血培养瓶检验结果与传统血培养瓶比较,差异无统计学意义(P>0.05)。结论自制负压血培养瓶采集血液时可与其他检验项目的负压管一起,一次穿刺,可减少二次损伤和污染,在临床病原微生物检验中具有现实的临床价值。
Objective To develop a practical negative pressure blood culture bottle,and to improve the efficacy of clinical pathogenic microorganism diagnosis and inspection.Methods The self-made negative pressure blood culture bottle adopted a 100 mL glass infusion bottle,which was composed of a bottle body and a bottle cap.The bottle body sealed by the bottle cap was in a negative pressure state.The temperature in the sealed bottle body was-10-65℃,the humidity was≤85%,and the pressure was-50--30 kPa.There was cellophane wrapping the bottle mouth at the bottle mouth.The outside of the cellophane was wrapped with gauze.The bottle in the negative pressure state was provided with 30 mL blood culture medium.The formula of blood culture medium was debugged,disinfected and prepared under negative pressure according to the fourth edition of National Clinical Laboratory Procedures,and the clinical performance was verified.Results The self-made negative pressure blood culture bottle showed positive results at the lowest concentration level of all strains,with a positive coincidence rate of 100%.There was no significant difference between the self-made negative pressure blood culture bottle and the traditional blood culture bottle(P>0.05).Conclusion The self-made negative pressure blood culture bottle can be punctured once together with the negative pressure tubes of other inspection items,which can reduce the secondary injury and pollution rate.It has practical clinical value in clinical pathogenic microorganism inspection.
作者
李忠涛
瞿良
陶娜
依南温
LI Zhongtao;QU Liang;TAO Na;YI Nanwen(Department of Medical Engineering,the 920th Hospital of the Joint Service Support Force of the People′s Liberation Army of China,Kunming,Yunnan 650032,China;Department of Laboratory Medicine,the 920th Hospital of the Joint Service Support Force of the People′s Liberation Army of China,Kunming,Yunnan 650032,China)
出处
《现代医药卫生》
2023年第21期3641-3646,共6页
Journal of Modern Medicine & Health
基金
中国人民解放军联勤保障部队第九二〇医院科技项目(2020YGD09)。
关键词
病原微生物
负压
血培养瓶
微生物敏感性试验
Pathogenic microorganism
Negative pressure
Blood culture bottle
Microbial sensitivity inspection
