摘要
为研究华南地区采用传统AAO工艺和MBR工艺(典型工艺为AAOA-MBR)的城市污水处理厂(净化厂)的菌群结构及其差异,采用16 S rRNA基因扩增子测序技术对11座净化厂的22条生产线进行了菌群结构表征,系统分析了硝化细菌和反硝化细菌的相对丰度及其在不同工艺中的种群特征与差异,并与其他地区的菌群结构进行了综合对比.结果表明,AAOA-MBR工艺中氨氧化细菌(Ammonium oxidizing bacteria,AOB)Nitrosomonas的平均相对丰度为AAO工艺中的2倍;各生产线中亚硝酸盐氧化细菌(Nitrite Oxidizing Bacteria,NOB)Nitrospira的平均相对丰度普遍较高,在采用AAO-MBR工艺的净水厂中,其相对丰度可达到22.22%;反硝化细菌则在AAO工艺中得到较好的富集,如反硝化聚糖菌Ca.Competibacter,其平均相对丰度在AAO工艺中可达到2.04%,远高于采用AAOA-MBR工艺的净水厂.与其他地区相比,华南地区净水厂中AOB丰度较低,NOB丰度较高,反硝化菌的优势种群也存在显著差异.华南地区净水厂中NOB的高丰度对短程硝化-反硝化的实现提出了更高的挑战;反硝化菌群的多样性及差异则进一步强调了不同净水厂需根据各自的反硝化菌群组成优化反硝化碳源投加.
To investigate the differences in microbial community structure and influencing factors in urban wastewater treatment plants(WWTPs)in the South China,which using traditional AAO process and MBR processes(typical processes being AAOA-MBR),16S rRNA gene ampli con sequencing technology was used to characterize the bacterial community structure of 22 treatment lines in 11 WWTPs.The relative abundance and population characteristics of nitrifying bacteria and denitrifying bacteria in different processes were systematically analyzed and compared with microbial community structures in other regions.Results showed that the average relative abundance of ammonia-oxidizing bacteria(AOB)Nitrosomonas in the AAO-MBR process was twice that in the AAO process.The average relative abundance of nitrite-oxidizing bacteria(NOB)Nitrospira was generally higher in all treatment lines,especially in the AAO-MBR process-based WWTPs,which reached up to 22.22%.On the other hand,denitrifying bacteria were better enriched in the AAO process,such as denitrifying glycogen-accumulating organism Ca.Competibacter,with an average relative abundance of 2.04%,much higher than in the AAO-MBR processbased WWTPs.In comparison to other regions,the relative abundances of NOB were commonly much higher than AOB in WWTPs in South China,which raised higher challenges for achieving main-stream short-cut nitrification in WWTPs in South China.The diverse and distinct denitrifying community in different WWTPs in South China also suggested that each plant may have to develop their own type of external carbon sources and the addition strategies.
作者
唐霞
黄福
李碧清
林学然
邱光磊
TANG Xia;HUANG Fu;LI Biqing;LIN Xueran;QIU Guanglei(Guangzhou Sewage Purification Co.Ltd.,Guangzhou 510163,China;School of Environment and Energy,South China University of Technology,Guangzhou 510006,China)
出处
《给水排水》
CSCD
北大核心
2023年第10期44-52,共9页
Water & Wastewater Engineering
