摘要
目的 探讨细菌培养和涂片镜检在微生物检验中的临床应用价值。方法 回顾并整理2021年1月—2022年12月平邑县人民医院收集的84份微生物检验样本,包括痰液样本、血液样本、脑脊液样本等,均接受细菌培养和涂片镜检。分析涂片镜检的合格率、细菌检出率以及阳性样本检出情况。结果 84份标本中有合格标本77份(包括A类标本37份和B类标本40份),不合格标本(C类)7份,合格率为91.67%。84份标本中,检出64份致病菌或优势生长菌,阳性检出率为76.19%。37份A类标本中检出阳性标本32份,阳性检出率为86.49%;40份B类标本中检出阳性标本25份,阳性检出率为62.50%;7份C类标本中检出阳性标本3份,阳性检出率为22.86%。A类标本的阳性检出率明显高于B类和C类标本(86.49%比62.50%、42.86%,χ_(1^)(2)=5.752,P1=0.016;χ_(2)^(2)=6.887,P2=0.009)。64份阳性样本中,检出革兰阴性菌42份,占比为65.63%,检出革兰阳性菌18份,占比为28.13%,检出真菌4份,占比为6.24%。结论 细菌培养和涂片镜检联合应用能最大限度确保检验效果,但在具体应用时还是容易出现假阴性结果,针对此类问题要依据影响因素,采取有针对性的措施,以提升检验效果。
Objective To explore the clinical application value of bacterial culture and smear microscopy in microbiological examination Methods The 84 samples collected from Pingyi County People's Hospital from January 2021 to December 2022 were reviewed and organized,including sputum samples,blood samples,cerebrospinal fluid samples and so on,all of which underwent bacterial culture and smear microscopy.The qualification rate of smear microscopy,the bacterial detectable rate of smear microscopy,and the detection status of positive samples were analyzed.Results The research results showed that out of 84 specimens,77 samples were qualified,including 37 samples of Class A and 40 samples of Class B),and 7 samples were unqualified(which were Class C specimens),with a qualification rate of 91.67%.Out of 84 specimens,64 samples were detected for pathogenic bacteria or dominant growth bacteria,with a positive detectable rate of 76.19%.Out of 37 specimens of Class A,32 samples were positive,with a positive detectable rate of 86.49%.Out of 40 specimens of Class B,25 samples were positive,with a positive detectable rate of 62.50%.Three out of 7 specimens of Class C were positive,with a positive detectable rate of 22.86%.The detectable rate of Class A specimens was higher than those of Class B specimens and Class C specimens(86.49%vs.62.50%,42.86%,χ_(1)^(2)=5.752,P1=0.016;χ_(2)^(2)=6.887,P2=0.009).Among the 64 positive samples,42 samples were Gram negative bacteria,accounting for 65.63%,18 samples were Gram positive bacteria,accounting for 28.13%,and 4 samples were fungi,accounting for 6.24%.Conclusions The combination of bacterial culture and smear microscopy could maximize the effectiveness of the test,but in specific applications,false negatives and other situations are still prone to occur.Targeted measures should be taken based on the influencing factors to improve the test effectiveness.
作者
王甲银
Wang Jiayin(Department of Clinical Laboratory,Pingyi County People's Hospital,Linyi 273300,Shandong,China)
出处
《实用检验医师杂志》
2023年第3期250-253,共4页
Chinese Journal of Clinical Pathologist
关键词
细菌培养
涂片镜检
微生物检验
应用价值
阳性检出率
Bacterial culture
Smear microscopy
Microbiological examination
Application value
Positive rate
