摘要
从湖州、嘉兴、绍兴3市12家肉鸡养殖场采集粪便、土壤、污水共720份样品,经16s rRNA测序鉴定,K-B法测定耐药表型,PCR法鉴定耐药基因型与整合子,并通过共现网络分析大肠杆菌耐药性传播风险。分离得到的234株大肠杆菌对头孢唑啉、阿奇霉素、四环素与磺胺类抗生素有较高的耐药率,且嘉兴市分离株对其中17种抗生素的耐药率均高于其他两市。基因型鉴定结果表明,234株大肠杆菌中,aadA1的检出率最高为88.89%,其次分别为tet(A)、sul1、blaTEM,而在29种耐药基因中,嘉兴市分离株中有18种基因的检出率高于其他两市。Ⅰ型整合酶3市分离株中的检出率分别为28.38%(湖州),40.96%(嘉兴),32.47%(绍兴)。结果表明:嘉兴市分离株有相对较高的耐药率与耐药基因检出率,共现网络分析显示在嘉兴市大肠杆菌中qnrB与mph(A)、fosA与blaTEM、fosA与sul1、sul2与aadA1有较高的共传播风险。本研究调查了浙江省湖州、嘉兴、绍兴3市肉鸡养殖环境的耐药情况与传播风险,为保障动物性食品安全与特定耐药基因的重点监测提供理论依据。
720 samples of feces,soil and sewage were collected from 12 broiler farms in Huzhou,Jiaxing and Shaoxing cities in this study.234 isolates of Escherichia coli were identified by 16s rRNA sequencing,antibiotic-resistant phenotypes were determined by K-B(Kirby-Bauer),antibiotic-resistant genotypes and integrons were identified by PCR.The transfer risk of antibiotic resistance of Escherichia coli was analyzed by co-occurrence network.234 isolates of Escherichia coli were highly resistant to cefazolin,azithromycin,tetracycline and sulfonamides,and the resistance rate of isolates in Jiaxing to 17 antibiotics was higher than that in the other two cities.The results of genotype identification showed that the detection rate of aadA1 was the highest among the 234 strains of Escherichia coli,which was 88.89%,followed by tet(A),sul1 and blaTEM,respectively.Among the 29 antibiotic resistance genes,the detection rate of 18 genes in Jiaxing was higher than that in the other two cities.The detection rates of type I integrase in Huzhou,Jiaxing and Shaoxing were 28.38%,40.96%and 32.47%,respectively.The results showed that the antibiotic resistance rate and the detection rate of antibiotic resistance genes of isolates in Jiaxing were relatively high.Co-occurrence network analysis showed that there was a high risk of co-transfer of qnrB and mph(A),fosA and blaTEM,fosA and sul1,sul2 and aadA1 in E.coli in Jiaxing.In summary,this study investigated antibiotic resistance and transmission risk in three cities of Zhejiang Province,providing scientific guidance for ensuring animal food safety and monitoring key specific antibiotic resistance genes.
作者
宗帅州
辛丽娜
白佳琪
韩剑众
曲道峰
Zong Shuaizhou;Xin Lina;Bai Jiaqi;Han Jianzhong;Qu Daofeng(Zhejiang Gongshang University,Hangzhou 310018)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2023年第9期276-284,共9页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金面上项目(32172188)。
关键词
肉鸡
大肠杆菌
耐药基因
共现网络
broiler
Escherichia coli
resistance genes
co-occurrence network
