摘要
目的检测遗传学及生理学实验室蛋白2(LGP2)、维甲酸诱导基因I(RIG-I)和黑色素瘤分化相关基因5(MDA5)在手足口病(HFMD)患儿的表达水平,探索其在HFMD中可能的临床意义。方法选择2020年5月至2021年5月西安交通大学第二附属医院、西安市儿童医院和西安市中心医院就诊的50例HFMD患儿作为研究对象,并以同期同年龄段体检儿童20例作为对照组。HFMD患儿按病原学检测结果分为肠道病毒71(EV-A71)型和柯萨奇病毒A6(CV-A6)型,再根据疾病轻重程度分为轻症、重症患儿。比较并分析各组LGP2、RIG-I和MDA5的mRNA相对表达量及三者相关性。结果50例HFMD患儿中EV-A71型26例(轻症16例,重症10例),CV-A6型24例(轻症17例,重症7例)。HFMD组患儿与对照组儿童的年龄、性别差异均无统计学意义(P>0.05)。LGP2 mRNA在EV-A71型和CV-A6型HFMD患儿中的相对表达量分别为2.37(1.78,3.25)%和1.88(1.35,3.13)%,均低于对照组2.97(2.61,3.55)%,CV-A6型HFMD患儿与对照组间差异有统计学意义(Z=-2.310,P=0.021);RIG-I mRNA在EV-A71型和CV-A6型HFMD患儿中的相对表达量分别为9.95(7.79,14.62)%和9.78(7.04,15.83)%,均低于对照组18.47(13.00,21.07)%,差异均有统计学意义(均P<0.05);MDA5 mRNA在EV-A71型和CV-A6型HFMD患儿中的相对表达量分别为4.41(2.82,5.99)%和3.98(2.18,7.41)%,均低于对照组5.10(3.52,7.71)%,但差异均无统计学意义。LGP2、RIG-I和MDA5 mRNA在EV-A71型和CV-A6型HFMD患儿轻症、重症间表达水平差异均无统计学意义。LGP2、RIG-I和MDA5 mRNA表达量具有较高相关性(均P<0.001)。结论LGP2、RIG-I和MDA5 mRNA相对表达量在HFMD患儿中呈不同程度降低,且三者具有相关性。
Objective To detect the expression levels of laboratory of genetics and physiology 2(LGP2),retinoic acid inducible gene I(RIG-I)and melanoma differentiation associated gene 5(MDA5)in children with hand,foot and mouth disease(HFMD),and to explore their possible clinical significance in HFMD.Methods Fifty children with HFMD,who visited Second Affiliated Hospital of Xi′an Jiao Tong University,Xi′an Children′s Hospital and Xi′an Central Hospital from May 2020 to May 2021,were selected as the research subjects,and 20 children with physical examination at the same age during the same period were selected as the control group.Children with HFMD were divided into enterovirus 71(EV-A71)type and coxsackievirus A6(CV-A6)type according to the results of pathogen detection,and then divided into mild group and severe group according to the severity of the disease.The relative mRNA expression levels of LGP2,RIG-I and MDA5 in each group,and the correlation among the three proteins were compared and analyzed.Results Among 50 cases of HFMD,26 cases were EV-A71 type(16 cases were mild and 10 cases were severe)and 24 cases were CV-A6 type(17 cases were mild and 7 cases were severe).There was no significant difference in age and sex between HFMD group and control group(P>0.05).The relative expression levels of LGP2 mRNA in EV-A71 and CV-A6 HFMD cases were 2.37(1.78,3.25)%and 1.88(1.35,3.13)%,lower than that in control group[2.97(2.61,3.55)%].Only the difference between CV-A6 HFMD children and control group was statistically significant(Z=-2.310,P=0.021).The relative expression levels of RIG-I mRNA in EV-A71 and CV-A6 HFMD cases were 9.95(7.79,14.62)%and 9.78(7.04,15.83)%,lower than that in control group[18.47(13.00,21.07)%].The differences were all statistically significant(P<0.05).The relative expression levels of MDA5 mRNA in EV-A71 and CV-A6 HFMD cases were 4.41(2.82,5.99)%and 3.98(2.18,7.41)%,lower than that in control group[5.10(3.52,7.71)%],but the differences were not statistically significant.There were no significant differences in the relative expression levels of the three indicators between the mild and severe groups of children with EV-A71 or CV-A6 HFMD.The expression levels of LGP2,RIG-I and MDA5 mRNA were highly correlated(P<0.001).Conclusion The relative expression levels of LGP2,RIG-I and MDA5 mRNA in children with HFMD are decreased in different degrees than those in normal children.And there is a correlation among them.
作者
张萌
王沐淇
郝苗
王欣钰
刘晨瑞
陈媛
张玉凤
党双锁
邓慧玲
李亚萍
Zhang Meng;Wang Muqi;Hao Miao;Wang Xinyu;Liu Chenrui;Chen Yuan;Zhang Yufeng;Dang Shuangsuo;Deng Huiling;Li Yaping(Department of Infectious Diseases,Second Affiliated Hospital of Xi′an Jiao Tong University,Xi′an 710004,China;Department of Neurology,Xi′an Children′s Hospital,Xi′an 710003,China;The Second Department of Infectious Diseases,Xi′an Children′s Hospital,Xi′an 710003,China;Department of Pediatrics,Xi′an Central Hospital,Xi′an 710003,China)
出处
《中国小儿急救医学》
CAS
2023年第10期750-755,共6页
Chinese Pediatric Emergency Medicine
基金
国家自然科学基金青年项目(81701632)
陕西省自然科学基础研究计划资助项目(2022JQ-916)
陕西省重点产业创新链(群)-社会发展领域,重症手足口病发病机制与预警体系关键技术研究(2022ZDLSF01-05)
西安市创新能力强基计划-医学研究项目,重症手足口病早期诊断关键技术研发平台建设(21YXYJ006)。
关键词
手足口病
生理学实验室蛋白2
维甲酸诱导基因I
黑色素瘤分化相关基因5
固有免疫
Hand,foot and mouth disease
Laboratory of genetics and physiology 2
Retinoic acid inducible gene I
Melanoma differentiation associated gene 5
Innate immunity
