摘要
CUL家族成员在植物体内发挥着重要作用,但关于辣椒CUL基因家族的全基因组鉴定及其在非生物胁迫下的表达模式分析报道较少。本研究对辣椒CUL基因家族进行了全基因组鉴定和表达分析。结果表明,共有12个CaCUL家族基因被系统鉴定,编码序列(CDS)全长为777~2 952 bp,非均匀地分布在6条染色体上,系统进化树将CUL基因分为4个亚家族。保守结构域分析结果表明,所有CUL蛋白均含有Cullin结构域,除此之外,有8个成员含有Cullin-Nedd8结构域,CaCUL1.6蛋白含有NB-ARC结构域。亚细胞定位预测发现CaCUL均定位于细胞质、细胞核。CaCUL基因的启动子中有多种与激素和胁迫响应等相关的顺式作用元件。基因表达模式分析结果表明,在不同生长阶段的不同组织中CUL参与了辣椒植株的生长和发育;在非生物胁迫中,大部分CUL基因都具有应激反应。本研究为进一步解析CaCUL家族基因在辣椒生长发育和非生物胁迫应答中的作用机理奠定了基础。
CUL family members play an important role in plants,but there are few reports on the whole genome identification of CUL gene family and its expression pattern analysis under abiotic stress in pepper.In this study,the whole genome of CUL gene family in pepper was identified and its expression was analyzed.The results showed that,a total of 12 CaCUL family genes were systematically identified,and the total length of coding sequence(CDS)was 777-2952 bp,which distributed on six chromosomes unevenly.CUL genes were divided into four subfamilies by phylogenetic tree.Analysis of the conserved domain showed that,all CUL genes contained Cullin domain.In addition,eight members contained Cullin-Nedd8 domain,and CaCUL1.6 contained NB-ARC domain.Prediction of subcellular localization showed that,CaCUL were located in cytoplasm and nucleus.There are many cis-acting elements related to hormones and stress response in the promoter of CaCUL gene.Results of gene expression pattern showed that,CUL participated in the growth and development of pepper plants in different tissues at different growth stages.In abiotic stresses,most CUL genes showed stress response.This study can lay foundation for further analysis of the mechanism of CaCUL family genes in pepper growth and abiotic stress response.
作者
刘芳
段盼盼
魏敏
匡小妍
马艳
张涛
马玉虎
魏兵强
LIU Fang;DUAN Pan-pan;WEI Min;KUANG Xiao-yan;MA Yan;ZHANG Tao;MA Yu-hu;WEI Bing-qiang(College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China)
出处
《江苏农业学报》
CSCD
北大核心
2023年第6期1275-1285,共11页
Jiangsu Journal of Agricultural Sciences
基金
兰州市人才项目(2021-RC-65)
甘肃省重点研发项目(21YF5NA091)。
