摘要
前期研究中筛选出1株拮抗多种植物病原真菌的枯草芽孢杆菌(Bacillus subtilis)8-32,具有产吲哚乙酸(indoleacetic acid,IAA)的能力。为提升其IAA的产量,对该8-32菌株产IAA的发酵培养基组成和发酵培养条件进行优化。利用单因素试验确定培养基中最优碳源为蔗糖,氮源为蛋白胨,无机盐为NaCl;利用Plackett-Burman试验确定影响菌株8-32产IAA的3个显著因素,并利用最陡爬坡试验和Box-Behnken试验,确定3个显著因素的最适范围及最优配比,并通过响应面分析找到中心点。最终确定枯草芽孢杆菌8-32产IAA的最适发酵培养基构成及发酵条件如下:蔗糖50 g·L^(-1)、NaCl 0.3 g·L^(-1)、蛋白胨79.2 g·L^(-1)、发酵时间96 h、装液量56.17 mL·瓶^(-1)(250 mL三角瓶)、pH 7.0。发酵液中IAA含量由优化前的8.48 mg·L^(-1)提升至优化后的65.06 mg·L^(-1)。回归模型的预测值与实测值相差小于0.05%,表明模型预测值可靠,优化工艺可行。
In previous study, Bacillus subtilis strain 8-32 was screened to antagonize a variety of plant pathogenic fungi, and has the ability to produce indoleacetic acid(IAA). In order to improve the yield of IAA, the composition of fermentation medium and fermentation conditions of strain 8-32 were optimized in this study. Firstly, the single factor test showed that the optimal carbon source was sucrose, nitrogen source was peptone and inorganic salt was NaCl. Secondly, three significant factors affecting IAA production of strain 8-32 could be found by Plackett-Burman test. Finally, the steepest climbing test and Box-Behnken test were used to find the optimal range and ratio of the three significant factors, and the central point was found by response surface analysis. The optimum fermentation medium composition and fermentation conditions of B.subtilis 8-32 for IAA production were as follows: sucrose 50 g·L^(-1), NaCl 0.3 g·L^(-1), peptone 79.2 g·L^(-1), fermentation time 96 h, liquid volume 56.17 mL·flask^(-1)(250 mL flask), pH 7.0. The content of IAA in fermentation broth increased from 8.48 mg·L^(-1) before optimization to 65.06 mg·L^(-1) after optimization. The difference between the predicted value of regression model and the measured value was less than 0.05%. The predicted value of the model is reliable and the optimized process can be used.
作者
王冬云
高峰
刘笑稳
董欣宇
高同国
朱宝成
WANG Dongyun;GAO Feng;LIU Xiaowen;DONG Xinyu;GAO Tongguo;ZHU Baocheng(Hebei Engineering Research Center for Agricultural Waste Resource Utilization/College of Life Sciences,Hebei Agricultural University,Baoding 071000,China)
出处
《扬州大学学报（农业与生命科学版）》
CAS
北大核心
2023年第5期127-134,共8页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
河北省重点研发计划项目(20322907D)
河北省自然科学基金资助项目(C2021204053)
河北省省属高等学校基本科研业务费研究项目(KY2021047)。
关键词
枯草芽孢杆菌
吲哚乙酸
发酵条件优化
响应面分析
Bacillus subtilis
IAA
fermentation conditions optimization
response surface analysis
