摘要
为了建立高效液相色谱(high-performance liquid chromatography,HPLC)指纹图谱结合一测多评法(quantitation analysis of multi-components by single marker,QAMS)评价傣药方剂“黄氏蓝瘟康”(Dai medicine prescription Huang’s Lanwenkang,DMPHL)的质量,试验采用HPLC对11批DMPHL复方的成分进行分析,将所得色谱信息导入“中药色谱指纹图谱相似度评价系统”2012版进行分析,结合混合对照品色谱信息对共有峰进行指认,建立DMPHL的HPLC指纹图谱;以大黄酚为内参物,建立可同时测定绿原酸、盐酸巴马汀、盐酸小檗碱、大黄酸、对甲氧基肉桂酸乙酯、大黄素及大黄酚含量的一测多评法,同时用外标法(external standard method,ESM)验证一测多评法测定结果的准确性。结果表明:11批DMPHL的HPLC指纹图谱共有峰为24个,不同批次DMPHL指纹图谱的相似度均在0.900以上;以大黄酚为内参物计算出绿原酸、盐酸巴马汀、盐酸小檗碱、大黄酸、对甲氧基肉桂酸乙酯、大黄素的相对校正因子分别为2.0170,0.7885,0.8725,7.9264,2.7131,0.6992;一测多评法与外标法测定结果的RSD值均小于3.00%,两种方法的测定结果无明显差异。表明试验所建立的HPLC指纹图谱结合一测多评法适用于DMPHL的质量评价。
In order to establish a high-performance liquid chromatographic(HPLC)fingerprint combined with quantitative analysis of multi-components by single marker(QAMS)to evaluate the quality of Dai medicine prescription Huang's Lanwenkang(DMPHL),in this experiment,HPLC was used to analyze the components of 1I batches of DMPHL compounds;the obtained chromatographic information was imported into the "Chinese medicine chromatography fingerprint similarity evaluation system"2012 edition for analysis;the common peak identification was identified by the reference substances,and the HPLC fingerprint of DMPHL was established.Using chrysophanol as an internal reference,QAMS method was established which could simultaneously determine the contents of chlorogenic acid,palmatine hydrochloride,berberine hydrochloride,chrysophanic acid,ethyl p-methoxycinnamate,emodin and chrysophanol.At the same time,the external standard method(ESM)was used to verify the accuracy of the determination results of the QAMS method.The results showed that the HPLC fingerprints of 11 batches of DMPHL had a total of 24 peaks,and the similarity of DMPHL fingerprints of different batches was above 0.900.Chrysophanol was used as an intermal reference,the relative correction factors of chlorogenic acid,palmatine hydrochloride,berberine hydrochloride,chrysophanic acid,ethyl p-methoxycinnamate,emodin were calculated to be 2.0170,0.7885,0.8725,7.9264,2.7131 and 0.6992,respectively.The RSD value of the determination results of the QAMS method and the external standard method was less than 3.00%,and there was no obvious difference between the determination results of the two methods,which suggested that the HPLC fingerprint established in the experiment combined with QAMS method was suitable for the quality evaluation of DMPHL.
作者
邓招游
李宜航
俞静
尹翠云
唐德英
王钦晖
DENG Zhaoyou;LI Yihang;YU Jing;YIN Cuiyun;TANG Deying;WANG Qinhui(Yunnan Branch of Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Jinghong 666100,China;Yunnan Provincial Key Laboratory of Sustainable Utilization of Southern Medicine,Jinghong 666100,China;Yunnan Inspection Institute for Veterinary Drugs and Feeds,Kunming 650000,China)
出处
《黑龙江畜牧兽医》
北大核心
2023年第22期116-122,共7页
Heilongjiang Animal Science And veterinary Medicine
基金
云南省科技人才和平台计划项目(202105AG070011)
云南省基础研究专项(202201AT070284)。
关键词
指纹图谱
一测多评法
高效液相色谱
质量控制
黄氏蓝瘟康
fingerprint
quantitation analysis of multi-components by single marker(QAMS)
high-performance liquid chromatographic(HPLC)
qualitycontrol
Huang's Lanwenkang
