摘要
以杂交稻南粳9108种子为试验材料,研究了NaCl预处理对盐胁迫下水稻种子的发芽率、发芽势、发芽指数、活力指数、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性、抗坏血酸(AsA)含量和还原型谷胱甘肽(GSH)含量的影响。结果显示,与对照组相比,0.1%NaCl试验组种子的发芽势、发芽率、发芽指数和活力指数分别增加了12.2%、14.6%、58.3%和120.8%,MDA含量减少了38.7%,SOD、CAT活性分别增加了65.2%、50.2%,AsA、GSH含量分别增加了113.2%、47.1%,差异均达到显著水平(P<0.05)。POD活性比对照组有所增加,但无显著差异。0.2%NaCl试验组种子上述发芽指标、保护酶活性、还原性物质含量明显低于对照组和0.1%NaCl试验组,而MDA含量则较对照组和0.1%NaCl试验组显著增加。结果表明,0.1%NaCl预处理可能通过提高种子萌发期的抗氧化能力,抑制脂质过氧化产物的过度积累,从而促进种子在盐胁迫下的萌发,为幼苗的正常生长发育奠定良好的基础。
The effects of NaCl pretreatments on germination percentage,germination energy,germination index and vigor index,malondialdehyde(MDA)content,activities of superoxide dismutase(SOD),peroxidase(POD)and cata⁃lase(CAT),ascorbate(AsA)and reduced glutathione(GSH)contents of hybrid rice Nanjing 9108 seeds under salt stress were studied.The results showed that,compared with the control group,the germination energy,germination rate,germination index and vigor index of seeds in 0.1%NaCl experimental group increased by 12.2%,14.6%,58.3%and 120.8%,the activities of SOD and CAT increased by 65.2%and 50.2%,and the contents of AsA and GSH increased by 113.2%and 47.1%under salt stress,respectively.And the differences between the control group and 0.1%NaCl experimental group reached a significant level(P<0.05).The POD activity was higher than that of the control group,but there was no significant difference.On the other hand,the above germination indexes,protec⁃tive enzymes activities and protective agent contents in 0.2%NaCl experimental group were significantly lower than those in control group and 0.1%NaCl experimental group under salt stress,while MDA content was significantly higher than both of groups.These results indicated that 0.1%NaCl pretreatments maybe improve the antioxidant ca⁃pacity of seeds during germination period,inhibited the excessive accumulation of lipid peroxidation products,and then promoting seed germination under salt stress,which laid a good foundation for the normal growth and develop⁃ment of seedlings.
作者
刘少华
朱学伸
王晗
闫敏
LIU Shaohua;ZHU Xueshen;WANG Han;YAN Min(School of Life Sciences,Chemistry and Chemical Engineering,Jiangsu Second Normal University,Nanjing 210013,China)
出处
《东北农业科学》
2023年第5期12-16,共5页
Journal of Northeast Agricultural Sciences
基金
国家自然科学基金项目(31700280)
江苏省自然科学基金项目(BK20170756)。
关键词
水稻
预处理
种子萌发
盐胁迫
抗氧化
Oryza sativa L.
Pretreatments
Seed germination
Salt stress
Antioxidant
