摘要
目的以41种梅州客家民间常用中草药为研究对象,评价其对α-葡萄糖苷酶和α-淀粉酶的抑制活性,并对活性最强的品种进行酶抑制类型探究。方法以半数抑制率(IC 50值)为指标,采用对-硝基苯基-α-D-吡喃葡萄糖苷(pNPG)法、3,5二硝基水杨酸(DNS)法评价41种梅州客家常用中草药95%乙醇提取物对α-葡萄糖苷酶和α-淀粉酶的抑制活性,采用酶促动力学方法与Lineweaver-Burk曲线分析活性最强品种对酶的抑制作用类型。结果40种常用中草药具有α-葡萄糖苷酶抑制活性,23种具有α-淀粉酶抑制活性,其中九节(Psychotria asiatica)表现出最强的抑制活性,其抑制α-葡萄糖苷酶、α-淀粉酶IC_(50)值分别为(0.17±0.001)、(0.09±0.001)mg·mL^(-1),抑制类型分别为可逆竞争性抑制和可逆非竞争性抑制。结论梅州客家常用中草药九节对糖代谢酶活性具有显著的抑制作用,在糖尿病的防治方面具有进一步研究和开发的潜在价值。
Objective Taking 41 kinds of Chinese herbal medicines commonly used in Meizhou Hakka as the research object,their inhibitory activities againstα-glucosidase andα-amylase were screened and the enzyme inhibition types of the species with the strongest activities were explored.Methods The inhibitory activities of 41 commonly used Hakka herbs in Meizhou againstα-glucosidase andα-amylase were evaluated by the p-Nitrophenyl a-D-mannopyranoside(pNPG)method and the 3,5-Dinitrosalicylic acid(DNS)method,using the inhibitory rate of half(IC_(50))as an index.The inhibitory activity of 95%ethanol extracts of 41 Chinese herbal medicines commonly used in Meizhou Hakka onα-glucosidase andα-amylase were analysed.The enzymatic kinetics method and Lineweaver-Burk curve were used to analyze the inhibitory type of the most active species.Results The results showed that 40 Chinese herbal medicines commonly used in Meizhou Hakka hadα-glucosidase inhibitory activity,and 23 medicines hadα-amylase inhibitory activity,among which Psychotria asiatica Wall.showed the strongest inhibitory activity with the IC 50 values aganistα-glucosidase andα-amylase of 0.17±0.001 mg·mL^(-1)and 0.09±0.001 mg·mL^(-1),respectively.The inhibition types were reversible competitive inhibition and reversible non-competitive inhibition,respectively.Conclusion The Psychotria asiatica Wall.Chinese herbal medicines commonly used in Meizhou Hakka has significant inhibitory effect on the activity of glucose metabolism enzymes,which has potential value for further research and development on the prevention and treatment of diabetes mellitus.
作者
丘建媚
刘付薇
陈星梅
庄远杯
张声源
吴俊标
QIU Jianmei;LIU Fuwei;CHEN Xingmei;ZHUANG Yuanbei;ZHANG Shengyuan;WU Junbiao(The Second Clinical Medical Colletge,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510006,China;Depertment of Pharmacy,Meizhou Hospital of Traditional Chinese Medicine,Meizhou 514071,China;Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas,Jiaying University,Meizhou 514015,China;Department of Pharmacy,The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,China)
出处
《医药导报》
CAS
北大核心
2023年第12期1758-1764,共7页
Herald of Medicine
基金
国家自然科学基金资助项目(8197140105)
广东省中医院拔尖人才项目(BJ2022KY15)
2022年广东省基地嘉应学院客家研究院大客家平台研究团队项目(22KYKT03)。
