摘要
目的探讨急性冠脉综合征(ACS)患者血清微小RNA-130a(miR-130a)、血管紧张素Ⅱ(AngⅡ)水平与冠状动脉病变程度的关系。方法选取2021年1月至2023年2月该院收治的ACS患者160例作为ACS组,按总Gensini评分将ACS患者分为轻度组(57例)、中度组(54例)、重度组(49例)。同期选取160例该院的体检健康者作为对照组。收集所有受试者临床资料,采用实时荧光定量PCR和酶联免疫吸附试验分别检测所有受试者血清miR-130a和AngⅡ水平;比较对照组、ACS组临床资料和血清miR-130a、AngⅡ水平,比较不同病变程度ACS患者血清miR-130a、AngⅡ水平;采用Pearson相关分析ACS患者血清miR-130a水平与AngⅡ的相关性;采用多因素Logistic回归分析影响ACS发生的危险因素;采用受试者工作特征曲线分析血清miR-130a、AngⅡ水平对中、重度ACS的诊断价值。结果与对照组比较,ACS组高血压史、糖尿病史比例及血清miR-130a、AngⅡ水平升高(P<0.05);轻度组、中度组、重度组ACS患者血清miR-130a、AngⅡ水平依次升高(P<0.05);ACS患者血清miR-130a水平与AngⅡ呈正相关(r=0.584,P<0.05);有高血压史、糖尿病史及血清miR-130a、AngⅡ水平升高是影响ACS发生的独立危险因素(P<0.05);血清miR-130a、AngⅡ及二者联合诊断中度ACS的曲线下面积(AUC)分别为0.728、0.823、0.885,二者联合诊断的AUC更高(P<0.05);血清miR-130a、AngⅡ及二者联合诊断重度ACS的AUC分别为0.731、0.730、0.825,二者联合诊断的AUC更高(P<0.05)。结论ACS患者血清miR-130a、AngⅡ水平较高,且血清miR-130a、AngⅡ水平均与ACS冠状动脉病变程度有关,二者联合对冠状动脉病变程度具有较高诊断价值。
Objective To investigate the relationship between serum levels of microRNA-130a(miR-130a),angiotensinⅡ(AngⅡ)and the degree of coronary artery lesions in patients with acute coronary syndrome(ACS).Methods A total of 160 ACS patients admitted to the hospital from January 2021 to February 2023 were selected as the ACS group.According to total Gensini score,ACS patients were divided into mild group(57 cases),moderate group(54 cases)and severe group(49 cases).At the same time,160 healthy people were selected as the control group.The clinical data of all subjects were collected.The serum levels of miR-130a and AngⅡwere detected by real-time fluorescence quantitative PCR and enzyme-linked immunosorbent assay,respectively.The clinical data and serum levels of miR-130a and AngⅡwere compared between control group and ACS group.The serum levels of miR-130a and AngⅡwere compared between ACS patients with different degrees of disease.Pearson correlation analysis was used to analyze the correlation between serum miR-130a level and AngⅡin ACS patients.Multivariate Logistic regression was used to analyze the risk factors of ACS.Receiver operating characteristic curve was used to analyze the diagnostic value of serum miR-130a and AngⅡlevels for moderate and severe ACS.Results Compared with the control group,the ACS group had significantly higher proportions of patients with a history of hypertension and diabetes and significantly higher serum levels of miR-130a and AngⅡ(P<0.05).The serum levels of miR-130a and AngⅡwere increased sequentially in the mild group,moderate group and severe group(P<0.05).Serum miR-130a level was positively correlated with AngⅡlevel in ACS patients(P<0.05).Hypertension,diabetes history and elevated serum levels of miR-130a and AngⅡwere independent risk factors for ACS(P<0.05).The area under the curve(AUC)of serum miR-130a,AngⅡand their combination in the diagnosis of moderate ACS were 0.728,0.823 and 0.885,respectively,and the AUC of the combination of miR-130a and AngⅡwas higher than that of miR-130a,AngⅡ(P<0.05).The AUC of serum miR-130a,AngⅡand their combination in the diagnosis of severe ACS were 0.731,0.730 and 0.825,respectively.The AUC of the combination of miR-130a and AngⅡwas higher than that of miR-130a and AngⅡ(P<0.05).Conclusion ACS patients serum miR-130-a,AngⅡlevel is higher,and the serum miR-130a,AngⅡlevels are associated with the ACS degree of coronary artery lesions,the combination of the both degree of coronary artery lesions with high diagnostic value.
作者
冯天保
刘宏颖
方媛
张阳
闻志军
李臻
FENG Tianbao;LIU Hongying;FANG Yuan;ZHANG Yang;WEN Zhijun;LI Zhen(Department of Cardiovascular Medicine,Zunhua People′s Hospital,Tangshan,Hebei 064200,China)
出处
《国际检验医学杂志》
CAS
2023年第24期3001-3005,共5页
International Journal of Laboratory Medicine
基金
河北省医学科学研究课题计划(20221604)。
