摘要
目的研究莲须Nelumbinis Stamen的化学成分。方法莲须甲醇提取物采用硅胶、C8反相柱、Sephadex LH-20、制备TLC、制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离得到21个化合物,分别鉴定为高根二醇(1)、高根二醇-3-醋酸酯(2)、2α-羟基齐墩果酸(3)、齐墩果酸(4)、4-epi-hederagenin(5)、4-epi-hederagenin methyl ester(6)、28α-同-β-香树脂醇乙酯(7)、白桦脂酸(8)、24-ketocholesterol(9)、7β-羟基谷甾醇(10)、7α-羟基谷甾醇(11)、3β-羟基豆甾-5-烯-7-酮(12)、(3β,5α,6β,22 E)-6-甲氧基麦角甾烷-7,22-二烯-3,5-二醇(13)、dankasterone A(14)、demethylincisterol A 3(15)、β-谷甾酮(16)、β-谷甾醇(17)、槲皮素(18)、异鼠李素(19)、山柰酚(20)、木犀草素(21)。结论化合物2、4~15为首次从莲属植物中分离得到,化合物16为首次从该植物中分离得到。
AIM To study the chemical constituents from Nelumbinis Stamen.METHODS The methanol extract from Nelumbinis Stamen was isolated and purified by silica gel,C8 reversed phase column,Sephadex LH-20,preparative TLC and preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Twenty-one compounds were isolated and identified as erythrodiol(1),erythordiol-3-acetate(2),2α-hydroxy-oleanolic acid(3),oleanolic acid(4),4-epi-hederagenin(5),4-epi-hederagenin methyl ester(6),28α-homo-β-amyrin acetate(7),betulinic acid(8),24-ketocholesterol(9),7β-hydroxy-sitosterol(10),7α-hydroxy-sitosterol(11),3β-hydroxy-stigmast-5-en-7-one(12),(3β,5α,6β,22 E)-6-methoxyergosta-7,22-diene-3,5-diol(13),dankasterone A(14),demethylincisterol A 3(15),β-sitostenone(16),β-sitosterol(17),quercetin(18),isorhamnetin(19),kaempferol(20),luteolin(21).CONCLUSION Compounds 2,4-15 are isolated from genus Nelumbo for the first time.Compound 16 is isolated from this plants for the first time.
作者
李玥
吴弢
LI Yue;WU Tao(Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处
《中成药》
CAS
CSCD
北大核心
2023年第12期3987-3994,共8页
Chinese Traditional Patent Medicine
基金
国家自然科学基金资助项目(81973475)。
关键词
莲须
化学成分
分离鉴定
Nelumbinis Stamen
chemical constituents
isolation and identification
