摘要
参加中国检验检疫科学研究院编号为ACAS-PT1343(2022)的能力验证,该能力验证考核能否从人工污染的样品中检出单核细胞增生李斯特菌检验。对随机人工污染样品22-D378、22-G483进行单核细胞增生李斯特菌检验,采用国标法、酶联免疫法和荧光定量PCR技术3种检测方法,探讨食品中单核增生李斯特菌的快速检测及鉴定。试验结果表明,编号22-D378样品检出单增李斯特菌,22-G483未检出。在国标方法的基础上,辅助酶联免疫和荧光定量PCR技术进行检测,缩短了检测时间,特异性和敏感性均较好,3种方法互相补充可确保试验结果的准确性。
To participate in the capability validation of the Chinese Academy of Inspection and Quarantine Sciences,numbered ACAS-PT1343(2022),to assess whether Listeria monocytogenes can be detected from artificially contaminated samples.Randomly contaminated samples 22-D378 and 22-G483 were tested for Listeria monocytogenes.Three detection methods were used,including national standard method,Mini VIDAS,and Applied Biosystems 7500fast PCR,to explore the rapid detection and identification of Listeria monocytogenes in food.Result number 22-D378 detected Listeria monocytogenes,while 22-G483 was not detected.Conclusion:On the basis of national standards,assisted enzyme-linked immunosorbent assay and fluorescence quantitative PCR technology can shorten the detection time,with good specificity and sensitivity.Combining these three methods can ensure the accuracy of experimental results.
作者
马力
沈锐
MA Li;SHEN Rui(Chongqing Yongchuan Institute for Food and Drug Control,Chongqing Yongchuan 402160,China)
出处
《食品工程》
2023年第4期44-46,64,共4页
Food Engineering
基金
重庆市永川区自然科学基金重点项目(2022ycjckx20022、2023yc-jckx20004)。
关键词
单核细胞增生李斯特菌
能力验证
鉴定
Listeria monocytogenes
proficiency testing
identification
