摘要
目的建立通关藤药材的HPLC指纹图谱,并结合化学计量学方法对不同产地通关藤质量进行评价。方法采用HPLC法建立指纹图谱,色谱柱为DiKMA C18(250 mm×4.6 mm,5μm),流动相为乙腈-0.05%磷酸水溶液,梯度洗脱,流速为1.0 ml/min,检测波长230 nm,柱温30℃,进样量10μl。将色谱信息导入中药色谱指纹图谱相似度评价系统(2012版),进行相似度分析。采用SPSS Statistics 26进行系统聚类分析,采用SIMCA 14.1软件进行主成分分析和偏最小二乘法判别分析(PLS-DA)。结果确定12个共有峰,指认其中2个色谱峰分别为通关藤苷G、通关藤苷I。15批样品指纹图谱与对照指纹图谱相似度为0.942~0.995。当平方欧式距离为20时,样品可聚为2类,S1~S3、S13~S15聚为一类,S4~S12聚为一类;主成分分析和PLS-DA结果表明,15批通关藤质量具有一定差异性,且与产地相关。结论该结果可为分析不同产地通关藤药材质量差异及后期相关配方颗粒的质量标准提供参考。
Objective To establish the HPLC fingerprint of MaRSDenia tenacissima;To evaluate the different origins of MaRSDenia tenacissima by combining chemometric methods.Methods High performance liquid chromatography(HPLC)method was adopted,with DiKMA C18 column(250 mm×4.6 mm,5μm),acetonitrile-0.05%phosphoric acid aqueous solution as mobile phase gradient elution,flow rate of 1.0 ml/min.The detection wavelength was set at 230 nm,the column temperature was 30℃,and sample size was 10µl.Chromatographic information was imported into the similarity evaluation system for TCM chromatographic fingerprints(2012 version)for similarity analysis.SPSS Statistics 26 was used for system clustering analysis,and SIMCA 14.1 software was used for principal component analysis and partial least squares discriminant analysis(PLS-DA).Results Totally 12 common peaks were identified.Two chromatographic peaks were identified as tenacissoside G and tenacissoside I.The relative similarity of fingerprints of 15 batches of samples and references ranged from 0.942 to 0.995.When the square Euclidean distance was 20,the samples could be grouped into two categories:S1-S3,S13-S15 were grouped into one category,and S4-S12 were grouped into another category.Principal component analysis(PCA)and partial least squares discriminant analysis(PLS-DA)showed that there were significant differences among 15 batches of MaRSDenia tenacissima,and there was a certain correlation with the origin.Conclusion The results can provide a reference for analyzing the differences of MaRSDenia tenacissima from different producing areas and the quality standards of related formula granules in the later stage.
作者
朱志坤
郭媛媛
王飘
王五兵
李思琪
陈凌云
Zhu Zhikun;Guo Yuanyuan;Wang Piao;Wang Wubing;Li Siqi;Chen Lingyun(College of Traditional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China)
出处
《国际中医中药杂志》
2023年第12期1549-1554,共6页
International Journal of Traditional Chinese Medicine
基金
云南省重大科技专项计划(202102AA310027)
2022年医疗服务与保障能力提升补助资金项目(Z155080000004)。
关键词
彝药
通关藤
指纹图谱
化学计量学
通关藤苷G
通关藤苷I
Yi drugs
MaRSDenia tenacissima
Fingerprint
Chemometrics
Tenacissoside G
Tenacissoside I
