摘要
旨在以犬乳腺肿瘤组织上分离所得的细胞CMT-N7为体外模型,评估常山酮和盐霉素联用对犬乳腺肿瘤细胞增殖、迁移和克隆形成的影响。采用常山酮、盐霉素单独以及两药联合处理,评估对犬乳腺肿瘤细胞的抑制作用,使用Chou-Talalay方法计算联合指数,确定最佳协同比例,进一步使用划痕试验和克隆形成试验评估两药在协同比例下联用对犬乳腺肿瘤细胞迁移和克隆形成能力的影响。结果显示:常山酮和盐霉素呈浓度依赖性地抑制CMT-N7细胞的增殖,且两药联用具有协同抑制作用,最佳协同比例为1∶1,该比例下两药联用对犬乳腺肿瘤细胞的迁移和克隆形成的抑制作用比单药更显著(P<0.01)。因此,常山酮和盐霉素联用能够协同抑制犬乳腺肿瘤细胞的增殖、迁移和克隆形成。本研究为兽医临床抗肿瘤药物的联合应用提供了依据。
In this study,the effects of the combination of genitone and salinomycin on the proliferation,migration and clonal formation of canine breast tumor cells were evaluated using CMT-N7 isolated from canine breast tumor tissue as an in vitro model.The combination of halofuginone,salinomycin and two drugs was used to evaluate the inhibitory effect of the two drugs on canine breast tumor cells,the combination index was calculated using the Chou-Talalay method to determine the optimal synergistic ratio,and scratch test and cloning formation test were further used to evaluate the effect of the combination of the two drugs on the migration and clonal formation abilities of canine breast tumor cells under the synergistic ratio.The results showed that the proliferation of CMT-N7 cells was inhibited in a concentration-dependent manner,the combination of the two drugs had a synergistic inhibitory effect,and the optimal synergistic ratio was 1∶1.At this ratio,the combination of the two drugs had a more significant inhibitory effect on the migration and clonal formation of canine breast tumor cells than that of a single drug(P<0.01).Therefore,the combination of halofuginone and salinomycin synergistically inhibited the proliferation,migration and clonal formation of canine breast tumor cells.This study might serve as a basis and reference for the combined application of veterinary clinical antitumor drugs in the future.
作者
胡诗恒
宋昕昊
林梦娟
郑玉玲
王俊棋
游凤
吕英军
高修歌
张军忍
江善祥
郭大伟
HU Shiheng;SONG Xinhao;LIN Mengjuan;ZHENG Yuling;WANG Junqi;YOU Feng;LÜYingjun;GAO Xiuge;ZHANG Junren;JIANG Shanxiang;GUO Dawei(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China)
出处
《畜牧与兽医》
CAS
北大核心
2024年第2期31-36,共6页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(32172918)
中央高校基本科研业务费项目(KYGD202002)。
关键词
常山酮
盐霉素
协同抑制
犬乳腺肿瘤细胞
halofuginone
salinomycin
synergistic inhibition
canine mammary tumor cell
