摘要
目的:优化AB-8型大孔树脂对花生红衣原花青素的纯化工艺并对纯化物组分进行表征鉴定。采用乳糜泻模型评价其对细胞毒性的抑制作用。研究方法:选用AB-8型大孔树脂,通过静态、动态吸附-解吸试验对纯化条件进行优化,用紫外-可见光谱、傅里叶红外和高效液相色谱串联质谱(UPLC-QTOF-MS/MS)对纯化物鉴定。基于乳糜泻细胞模型评价纯化物对细胞毒性的抑制作用。结果表明:纯化条件:上样质量浓度1.2 mg/mL,上样液pH值3.0,上样流速1.0 mg/mL,上样量360 mL,吸附时间180 min,解吸液为120 mL的40%乙醇溶液,解吸时间40 min,解吸流速1.0 mg/mL,此时测得该纯化物纯度达95%。紫外-可见光谱分析表明该纯化物属于原花青素类;傅里叶红外分析表明纯化物是以原花青定为主要结构单元,与标准品特征吸收峰趋势相符;UPLC-QTOF-MS/MS分析表明Pspc纯化物以A型原花青素为主要成分;细胞试验结果表明该纯化物在一定程度上可明显抑制乳糜泻模型的毒性作用。研究结果可为花生红衣资源综合开发提供理论参考。
Objective:To optimize the purification process of AB-8 macroporous resin for peanut red procyanidins and to characterize and identify the components of the purified product,and to evaluate its inhibitory effect on cytotoxicity in celiac disease model.Methods:AB-8 macroporous resin was selected,and the purification conditions were optimized by static and dynamic adsorption-desorption indexes.The purified material was analyzed and identified by Ultraviolet/visible spectroscopy,Fourier transform infrared spectroscopy and High performance liquid chromatography-tandem mass spectrometry(UPLC-QTOF-MS/MS).The inhibitory effect of purified material on cytotoxicity was evaluated based on celiac disease cell model.Results:Purification conditions:The adsorption time was 180 min.The desorption solution was 120 mL 40%ethanol solution.The desorption time was 40 min.The desorption flow rate was 1.0 mg/mL.Ultraviolet/visible spectrum analysis showed that the purified substance belonged to proanthocyanidins.Fourier infrared analysis showed that protocyanin was the main structural unit of the purified product,which was consistent with the characteristic absorption peak trend of the standard product.UPLC-QTOF-MS/MS analysis showed that type A procyanidins were the main component of Pspc purified products.The results of cell test showed that the purified substance could significantly inhibit the toxicity of celiac disease model to some extent.This study can provide some theoretical reference for comprehensive development of peanut red resources.
作者
王娜
郑玉茹
徐超
李军伟
任红涛
余秋颖
Wang Na;Zheng Yuru;Xu Chao;Li Junwei;Ren Hongtao;Yu Qiuying(College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450046;College of Food Science and Technology,Henan Agricultural University,Zhengzhou 450002;Longhu Laboratory,Zhengzhou 450046;International Joint Research Center of Animal Immunology,Henan Agricultural University,Zhengzhou 450046;Zhengzhou Key Laboratory of Nutrition and Health Food,Zhengzhou 450002)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2023年第12期167-178,共12页
Journal of Chinese Institute Of Food Science and Technology
基金
河南省创新生态支撑专项(HARS-22-05-Z1)。
