摘要
目的:通过黏膜下注射槟榔碱溶液构建SD大鼠口腔黏膜下纤维化(OSF)模型。方法:将20只SD大鼠随机分为实验组和对照组。实验组大鼠采用口腔颊黏膜下注射槟榔碱溶液进行造模,对照组大鼠则注射生理盐水。处理10周后,观察大鼠口腔黏膜颜色及体质量变化,测量被动开口度;苏木素-伊红(HE)染色、Masson染色观察黏膜组织病理学变化;免疫组化染色和实时荧光定量PCR检测黏膜组织内平滑肌肌动蛋白(α-SMA)、血小板-内皮细胞黏附分子(PECAM-1/CD31)蛋白及基因表达水平。结果:实验组大鼠颊黏膜出现白色病变,张口度减小(P<0.001);大鼠颊黏膜上皮萎缩,固有层胶原纤维沉积;大鼠黏膜组织内α-SMA蛋白和基因表达显著增加(P<0.001),CD31蛋白和基因表达显著减少(P<0.001)。结论:槟榔碱黏膜下注射法可构建出具有OSF典型症状、病理变化符合OSF的大鼠模型。
Objective:To construct a sprague dawley(SD)rat oral submucosal fibrosis model by injecting arecoline solution under the mucosa.Methods:20 SD rats were randomly divided into an experimental group and a control group.The rats were injected with arecoline solution under the buccal mucosa for modeling in experimental group,while the rats were injected with physiological saline in control group.After 10 weeks of treatment,the oral mucosa color and weight of rats were observed,and the passive mouth opening was measured;hematoxylin-eosin(HE)and Masson staining were used to observe pathological changes in mucosal tissue;Immunohistochemical staining and real-time fluorescence quantitative PCR were used to detect the protein and gene expression levels ofα-Smooth muscle action(α-SMA)and platelet endothelial cell adhesion molecule-1(PECAM-1/CD31)in mucosal tissue.Results:In experimental group,white lesions appeared on the buccal mucosa of the rats,and the mouth opening decreased(P<0.001);epithelial atrophy and deposition of collagen fibers in the lamina propria appeared in mucosal tissue;protein and gene expression levels ofα-SMA significantly increased(P<0.001),while the expression levels of CD31 significantly decreased in mucosal tissue(P<0.001).Conclusions:The submucosal injection method of arecoline can construct an OSF rat model with typical symptoms and significant pathological changes.
作者
郭振兴
王悦
许昊清
王舒妍
焦凯
GUO Zhenxing;WANG Yue;XU Haoqing;WANG Shuyan;JIAO Kai(Department of Prosthodontics,School of Stomatology,The Fourth Military Medical University,Xi'an 710032,China;State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,National Clinical Research Center for Oral Diseases,Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture,Xi'an 710032,China;Department of Stomatology,Tangdu Hospital,The Fourth Military Medical University,Xi'an 710032,China)
出处
《口腔生物医学》
2024年第1期21-25,37,共6页
Oral Biomedicine
基金
国家自然科学基金(81870787,82170978)
陕西省杰出青年科学基金(2021JC-34)。
