摘要
以来源于珞红4A的Nramp5基因缺失突变型、来源于lcd1的Nramp5基因点突变型及缺失与点突变的杂合体为材料,比较在有效镉(Cd)含量为0.58 mg/kg(pH值5.3)的中度污染稻田种植条件下不同突变类型和遗传背景材料的糙米Cd和锰(Mn)含量。结果表明,遗传背景不影响由Nramp5基因缺失或点突变所赋予的糙米Cd和Mn低积累特性;Nramp5基因缺失突变与点突变的杂合体的糙米Cd含量为0.082mg/kg,显著低于含野生型Nramp5基因的对照品种9311(2.345 mg/kg),且与Nramp5基因缺失或点突变的纯合体相比,Cd含量和Mn含量均无显著差异;Nramp5基因缺失纯合体、点突变纯合体及缺失与点突变杂合体的糙米Cd含量均符合国家食品安全标准(≤0.2 mg/kg)。因此,来源于珞红4A的Nramp5基因缺失突变的亲本与来源于lcd1的Nramp5基因点突变的亲本配组,其杂交种仍然具有糙米Cd低积累特征,本研究为杂交水稻低镉积累分子育种提供了重要参考数据。
The derivatives of deletion mutation of Nramp5 gene from Luohong 4A,point mutation of Nramp5 gene from lcd1 and heterozygotes of deletion and point mutation of Nramp5 gene in rice were used to characterized the contents of cadmium(Cd)and manganese(Mn)in brown rice,which were of different genetic backgrounds and planted in cadmium-contaminated paddy fields(0.58 mg/kg available Cd at pH 5.3).The results showed that the genetic background did not affect the low accumulation characteristics of Cd and Mn in brown rice caused by deletion or point mutation of Nramp5 gene.The average Cd content in brown rice with heterozygotes of deletion and point mutation of Nramp5 gene was 0.082 mg/kg,which was significantly lower than the control variety 9311 containing wild-type Nramp5 gene(2.345 mg/kg),and there was no significant difference in Cd and Mn content compared with that of the homozygotes of deletion or point mutation of Nramp5 gene,respectively.All of the Cd contents in brown rice of deletion mutation homozygote,point mutation homozygote and heterozygote of deletion and point mutation were lower than the threshold of the national food safety standard(≤0.2 mg/kg).The results indicated that the hybrid still had the characteristics of low Cd accumulation in brown rice even though one parental line was of Nramp5 gene deletion mutation from Luohong 4A and the other parental line was of Nramp5 gene point mutation from lcd1,which provides important reference data for molecular breeding of low Cd accumulation in hybrid rice.
作者
宋嘉俊
翁绿水
李锦江
邓力华
王学华
肖国樱
SONG Jiajun;WENG Lüshui;LI Jinjiang;DENG Lihua;WANG Xuehua;XIAO Guoying(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China;Institute of Subtropical Agriculture,Chinese Academy of Sciences/Key Laboratory of Agro-ecological Processes in Subtropical Region,Changsha,Hunan 410125,China)
出处
《杂交水稻》
CSCD
北大核心
2024年第1期17-26,共10页
Hybrid Rice
基金
海南省崖州湾种子实验室与中国种子集团有限公司联合揭榜挂帅项目。
关键词
水稻
糙米
镉
锰
Nramp5基因
突变
低镉积累
rice
brown rice
cadmium
manganese
Nramp5 gene
mutation
low Cd accumulation
