摘要
本试验运用正交试验法对优化艾叶黄酮的提取工艺及抗氧化活性进行了初步探究。经过试验得出,艾叶黄酮的最佳提取工艺为80%乙醇溶液浸取1∶25(g/mL)料液比的艾叶粉末4 h并超声提取50 min,提取率为3.53%。艾叶黄酮提取液浓度与总还原能力呈正相关关系,当艾叶黄酮质量浓度为1 mg/mL时,对DPPH·和·OH的清除率分别为96.35%和97.49%,具有较强的抗氧化活性。
This experiment uses orthogonal design to optimize the extraction process of flavonoids from Artemisia argyi leaves and preliminarily explore their antioxidant activity.After experiments,it was found that the optimal extraction process for flavonoids from Artemisia argyi leaves is to soak the powder of Artemisia argyi leaves with a ratio of 1∶25(g/mL)in 80%ethanol solution for 4 hours and extract it with ultrasound for 50 minutes,with an extraction rate of 3.53%.There is a positive correlation between the concentration of Artemisia argyi flavonoid extract and total reducing ability.When the concentration of Artemisia argyi flavonoid is 1 mg/mL,the clearance rates of DPPH·and·OH are 96.35%and 97.49%,indicating strong antioxidant activity.
作者
张小凤
曾颖欣
周丽萍
黄丽华
凌海燕
ZHANG Xiaofeng;ZENG Yingxin;ZHOU Liping;HUANG Lihua;LING Haiyan(Zhaoqing University,Zhaoqing 526061,China)
出处
《工业微生物》
CAS
2024年第1期134-136,共3页
Industrial Microbiology
基金
肇庆市科技计划项目“艾叶黄酮提取工艺优化和抗菌、抗氧化活性研究”(2022040303004)
肇庆学院校级项目“柑橘还原糖检测方法的评价和优化”(QN202234)。
关键词
艾叶
黄酮
提取工艺
抗氧化活性
artemisia argyi leaves
flavonoids
the optimal extraction process
antioxidant activity.
