摘要
皮肤是人体内非常复杂的器官,构建仿生的皮肤模型一直是是临床和研究中的热点与难点。3D生物打印技术的出现解决了皮肤构建物的问题,它可以通过定制皮肤形状,精确分配细胞和其他材料,实现快速可靠的仿生皮肤替代品生产,满足临床和工业要求。此外,它具有优异的性能、灵活性、高分辨率、重现性和高通量,可以用于药物筛选、化妆品测试以及皮肤移植等,并且可以为更多烧伤或糖尿病患者提供有效的皮肤替代物。结合干细胞的3D生物打印技术给研究和医疗专业人员带来更多解决皮肤疾病的方案。多能干细胞是有吸引力的候选细胞,因为,它们除了在伦理上被接受外,还可以根据患者的情况进行基因定制,实现具有预定结构的细胞载体,可以纳入血管网络、毛囊和汗腺等仿生层次的结构,以提高组织功能和美学植入。该文主要综述3D生物打印技术进行皮肤构建的研究现状,重点讨论了不同3D生物打印工艺构建皮肤的特点,并剖析了含有附属器的皮肤构建物下游应用需求,为本领域在体外生理或病理模型构建、再生医学和临床等研究提供参考。
The skin,being a highly intricate organ within the human body,has long posed a challenging and significant aspect in both clinical and research domains when constructing a bionic skin model.3D bioprinting technology has effectively addressed this issue by facilitating the creation of skin constructs.This innovative technique allows for the rapid and dependable production of bionic skin substitutes,as it enables the customization of skin shapes and the precise distribution of cells and other materials to meet the specific demands of clinical and industrial applications.Furthermore,this technology exhibits exceptional performance,adaptability,superior resolution,reproducibility,and high throughput,rendering it suitable for diverse applications such as drug screening,cosmetic testing,and skin grafts.Consequently,it holds the potential to serve as a viable skin substitute for a larger population of individuals afflicted with burns or diabetes.The integration of 3D bioprinting technology with stem cells has provided researchers and medical professionals with increased opportunities to address skin diseases.Pluripotent stem cells are particularly promising due to their ethical acceptability and ability to be genetically customized to meet individual patient’s needs.This customization enables the development of cell carriers with predetermined structures,which can incorporate biomimetic hierarchies,including blood vessel networks,hair follicles,and sweat glands.These advancements aim to enhance tissue functionality and improve the aesthetic outcomes of implantation procedures.This article summarizes the current research progress in skin construction using 3D bioprinting technology.It specifically highlights the distinctive features of skin construction achieved through various 3D bioprinting techniques.Additionally,it analyzes the specific requirements for skin construction that includes appendages,aiming to offer valuable insights for the development of in vitro physiological or pathological models,regenerative medicine,and clinical research within this domain.
作者
李玲
丁文
刘明
LI Ling;DING Wen;LIU Ming(Clinical Laboratory,Hei Long Jiang Provincial Hei He Center For Disease Control And Prevention,Heihe 164300,Heilongjiang,China;Institute of Material Forming and Control Engineering,College of Materials Science and Engineering,Zhejiang University of Technology,Hangzhou 310014,China;Lab of Research and Engineering of Cell Therapy Technology,Hangzhou Institute of Medicine(HIM),Chinese Academy of Sciences,Zhejiang Cancer Hospital,Hangzhou 310022,China)
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2024年第2期198-207,共10页
Chinese Journal of Biochemistry and Molecular Biology
基金
浙江省自然基金/重点项目(No.LZ22A020005)资助。
