摘要
以广东新会生产的广陈皮作为原材料,利用超声波辅助提取,采用正交试验法优化广陈皮黄酮的提取工艺,并进行抗氧化活性的测定。以芦丁为参照品,采用紫外可见光分光光度法建立了测定广陈皮中黄酮含量的定量分析方法。采取正交试验法,在单因素试验的基础上,分析了乙醇浓度、超声时间、超声功率、固液比这4个因素的交互作用,得到最佳条件并且进行验证。最后采用清除DPPH自由基和清除羟自由基的方法研究广陈皮黄酮的抗氧化能力。得出结论:当乙醇浓度为60%、超声时间为45 min、超声功率为300 W、固液比为1∶50时,广陈皮黄酮提取率可达到1.86%。随着黄酮浓度的增大,DPPH自由基和羟自由基的清除率越高,证明了广陈皮黄酮的抗氧化能力较高,是一种强抗氧化剂。
With Cantonese tangerine peel produced in Xinhui,Guangdong as the raw material,the ultrasonic-assisted extraction process of flavonoids from Cantonese tangerine peel is optimized by orthogonal test method,and the antioxidant activity is determined.With rutin as the reference,a quantitative analysis method for the determination of flavonoid content in Cantonese tangerine peel is established by ultraviolet and visible spectrophotometry.Based on single factor test,orthogonal test method is used to analyze the interaction of ethanol concentration,ultrasonic time,ultrasonic power and solid-liquid ratio,so as to obtain the best conditions and verify them.Finally,the antioxidant capacity of flavonoids from Cantonese tangerine peel is studied by the method of scavenging DPPH free radicals and hydroxyl free radicals.It is concluded that when the ethanol concentration is 60%,the ultrasonic time is 45 min,the ultrasonic power is 300 W and the solid-liquid ratio is 1∶50,the extraction rate of flavonoids from Cantonese tangerine peel could reach 1.86%.With the increase of flavonoid concentration,the scavenging rates of DPPH and hydroxyl free radicals are higher,indicating that the flavonoids from Cantonese tangerine peel have higher antioxidant capacity and they are a strong antioxidant.
作者
唐婷范
徐紫薇
李霞清
田玉红
李雪松
程昊
TANG Ting-fan;XU Zi-wei;LI Xia-qing;TIAN Yu-hong;LI Xue-song;CHENG Hao(Guangxi Key Laboratory of Green Processing of Sugar Resources,College of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006,China)
出处
《中国调味品》
CAS
北大核心
2024年第3期15-19,共5页
China Condiment
基金
广西自然科学基金面上项目(2021GXNSFAA220067,2022GXNSFAA035490)。
关键词
广陈皮
黄酮
超声波辅助提取
正交试验法
抗氧化
Cantonese tangerine peel
flavonoids
ultrasonic-assisted extraction
orthogonal test method
antioxidation
