摘要
为探究稀土元素铈(Ce)对锌(Zn)胁迫下小麦幼苗生长的缓解效应,通过水培试验,以百农307为试验材料,研究外源Ce对500μmol·L^(-1)Zn胁迫下小麦幼苗生长、Zn积累和生理特性的影响.结果表明,500μmol·L^(-1)Zn胁迫显著抑制了小麦幼苗叶绿素含量(叶绿素a、叶绿素b和总叶绿素)、光合作用和生物量的积累,使得幼苗根系变短变粗、侧根减少,同时降低了超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)活性和可溶性蛋白含量,增加了丙二醛(MDA)的积累.外源Ce降低了根系对Zn的吸收和转运,缓解了Zn胁迫对小麦幼苗的毒害效应,具体表现为叶绿素含量和光合作用参数的升高;提高了抗氧化酶活性和可溶性蛋白含量并降低MDA质量摩尔浓度;降低脂质过氧化对细胞膜的损伤,最终表现为小麦幼苗根系和茎叶干物质量的增加.外源Ce可通过提高Zn胁迫下小麦幼苗的光合特征、抗氧化酶活性和渗透调节物质的方式,降低细胞膜脂质的过氧化程度,缓解Zn胁迫对小麦幼苗生长的毒害作用.
This research aimed to clarify the mitigative effect of exogenously applied rare earth element cerium(Ce)on the growth,zinc(Zn)accumulation,and physiological characteristics of wheat(Triticum aestivum L.)seedlings under Zn stress.The wheat variety studied was Bainong307(BN307),and Zn stress was achieved by growing seedlings in a hydroponic culture experiment with 500μmol·L^(-1) Zn^(2+)added to the culture solution.It was found that Zn stress at 500μmol·L^(-1) significantly inhibited the chlorophyll content,photosynthesis,and biomass accumulation of wheat seedlings.Seedling roots became shorter and thicker,and the lateral roots decreased under Zn stress.The Zn stress also increased MDA accumulation and the degree of cell membrane lipid peroxidation and reduced soluble protein contents and the activities of antioxidant enzymes such as superoxide dismutase(SOD),catalase(CAT),and ascorbate peroxidase(APX).On the contrary,exogenous Ce decreased the adsorption and transport of Zn by the root system and alleviated the damage of Zn stress to wheat seedlings.Specifically,the increase in chlorophyll content(chlorophyll a,chlorophyll b,and total chlorophyll)and photosynthetic parameters,the enhancement of antioxidant enzymes activities and soluble protein levels,and the reduction in MDA content and the damage of lipid peroxidation to the cell membrane were all driven by exogenous Ce,which ultimately led to the increase in dry matter biomass of the root system and shoot.In summary,these results provide basic data for the application of exogenous Ce to alleviate Zn toxicity to plants.
作者
张静静
徐正阳
焦秋娟
范丽娜
刘芳
赵颖
宋佳
化党领
李鸽子
柳海涛
ZHANG Jing-jing;XU Zheng-yang;JIAO Qiu-juan;FAN Li-na;LIU Fang;ZHAO Ying;SONG Jia;HUA Dang-ling;LI Ge-zi;LIU Hai-tao(College of Resources and Environment,Henan Agricultural University,Zhengzhou 450046,China;National Engineering Research Center for Wheat,Henan Agricultural University,Zhengzhou 450046,China)
出处
《环境科学》
EI
CAS
CSCD
北大核心
2024年第2期1141-1149,共9页
Environmental Science
基金
国家重点研发计划重点专项(2021YFD1700904)
河南省农业农村厅重点项目(30801910)
河南省留学人员科研择优资助项目(30602340)
河南农业大学大学生创新训练计划项目(2022DC158)。
关键词
铈(Ce)
锌(Zn)胁迫
小麦幼苗
生理特性
缓解效应
cerium(Ce)
zinc(Zn)stress
wheat seedlings
physiological characteristics
mitigative effect
