摘要
目的观察紫檀芪(PTE)调节自噬改善压力超负荷大鼠心肌肥厚的作用。方法将30只SD大鼠随机分为假手术组(sham组)、压力超负荷手术组[腹主动脉缩窄术(AAC)组]、AAC+PTE组,每组10只。除sham组外,其余各组均行AAC建立压力超负荷心肌肥厚模型。PTE通过灌胃处理,sham组给予生理盐水灌胃。术后观察4周,使用小动物超声仪测量室壁厚度、左室缩短分数(LVFS),蛋白免疫印迹实验(Western blot)检测自噬相关蛋白beclin1、微管相关蛋白1轻链3(LC3)表达及肥大信号分子细胞外调节蛋白激酶(ERK)的表达,免疫组化检测氧化应激指标8-OHdG的表达。结果AAC组室壁厚度、beclin1、LC3-Ⅱ/Ⅰ、p-ERK、8-OHdG均高于sham组,而LVFS低于sham组,差异均有统计学意义(P<0.05)。AAC+PTE组的室壁厚度、beclin1、LC3-Ⅱ/Ⅰ、p-ERK、8-OHdG均低于AAC组,而LVFS高于AAC组,差异均有统计学意义(P<0.05)。结论PTE能够改善ACC大鼠心肌肥厚,其机制可能与调节自噬、降低氧化应激有关。
Objective To observe the effect of pterostilbene(PTE)in regulating autophagy to improve myocardial hypertrophy in pressure overloaded rats.Methods Thirty SD rats were randomly divided into sham group,pressure overload group(abdominal aortic constriction[AAC]group),and AAC+PTE group,10 animals each group.Except for sham group,all other groups underwent AAC to establish a pressure overload myocardial hypertrophy model.PTE was treated by gavage,and saline gavage was given to sham group.Postoperative observation was performed for 4 weeks.Echocardiography was used to measure ventricular wall thickness and left ventricular fractional shortening(LVFS),Western blot was used to detect the expression of autophagy-related proteins beclin1,microtubule-associated protein 1 light chain 3(LC3)and the expression of hypertrophic signaling molecule extracellular regulated protein kinases(ERK),and immunohistochemistry was used to detect the expression of oxidative stress index 8-OHdG.Results Ventricular wall thickness,beclin1,LC3-Ⅱ/Ⅰ,p-ERK,and 8-OHdG in the AAC group were higher than those in the sham group,while LVFS was lower than that in the sham group,and the differences were statistically significant(P<0.05).Ventricular wall thickness,beclin1,LC3-Ⅱ/Ⅰ,p-ERK,and 8-OHdG in the AAC+PTE group were lower than those in the AAC group,while LVFS was higher than that of the AAC group,and the differences were statistically significant(P<0.05).Conclusion PTE can ameliorate myocardial hypertrophy in ACC rats,and the mechanism may be related to the regulation of autophagy and reduction of oxidative stress.
作者
杨资鉴
徐陶锐
王家璞
李慧
YANG Zijian;XU Taorui;WANG Jiapu;LI Hui(Department of Cardiology,Shanxi Cardiovascular Hospital,Shanxi Province,Taiyuan030024,China;Laboratory,Shanxi Cardiovascular Hospital,Shanxi Province,Taiyuan030024,China;Department of Encephalopathy,the Hospital of Shanxi University of Chinese Medicine,Shanxi Province,Taiyuan030024,China)
出处
《中国当代医药》
CAS
2024年第4期18-21,26,共5页
China Modern Medicine
基金
山西省应用基础研究计划自然科学基金项目(201901D111444)
山西省心血管病医院科研激励计划项目(XYS20180101)。
关键词
紫檀芪
自噬
压力超负荷
心肌肥厚
大鼠
Pterostilbene
Autophagy
Pressure overload
Myocardial hypertrophy
Rat
